In Vitro Imaging of Thyroid Tissues Using Two-Photon Excited Fluorescence and Second Harmonic Generation

被引：8

作者：

Huang, Zufang ^{[1
]}

Li, Zuanfang ^{[1
]}

Chen, Rong ^{[1
,2
]}

Lin, Juqiang

Li, Yongzeng

Li, Chao ^{[3
]}

机构：

[1] Fujian Normal Univ, Key Lab OptoElect Sci & Technol Med, Minist Educ, Fuzhou, Peoples R China

[2] Xiamen Univ, State Key Lab Phys Chem Solid Surface, Xiamen, Peoples R China

[3] Fujian Prov Tumor Hosp, Fuzhou, Peoples R China

来源：

PHOTOMEDICINE AND LASER SURGERY | 2010年 / 28卷

基金：

中国国家自然科学基金;

关键词：

MULTIPHOTON MICROSCOPY; AUTOFLUORESCENCE; ACCUMULATION; COLLAGEN;

D O I：

10.1089/pho.2009.2563

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Objective: To evaluate the feasibility of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging to discriminate the normal, nodular goiter and papillary cancerous thyroid tissue. Materials and Methods: In total, 45 fresh thyroid specimens (normal, 15; nodular goiter, 12; and papillary cancerous, 18) from 31 subjects were directly imaged by the TPEF and SHG combination method. Results: The microstructure of follicle and collagen structure in thyroid tissue were clearly identified, morphologic changes between normal, nodular goiter, and papillary cancerous thyroid tissue were well characterized by using two-photon excitation fluorescence. SHG imaging of the collagen matrix also revealed the differences between normal and abnormal. Conclusions: Our preliminary study suggests that the TPEF and SHG combination method might be a useful tool in revealing pathologic changes in thyroid tissue.

引用

页码：S129 / S133

页数：5

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