Characterization of reproductive proteins in the Mexican fruit fly points towards the evolution of novel functions

被引:14
作者
Cordova-Garcia, Guadalupe [1 ]
Esquivel, Carlos J. [2 ]
Perez-Staples, Diana [1 ]
Ruiz-May, Eliel [3 ]
Herrera-Cruz, Mariana [4 ]
Reyes-Hernandez, Martha [5 ]
Abraham, Solana [6 ]
Aluja, Martin [3 ]
Sirot, Laura [7 ]
机构
[1] Univ Veracruzana, INBIOTECA, Ave Culturas Veracruzanas 101,Col E Zapata, Xalapa 91090, Mexico
[2] Bayer CropScience, R&D Seeds & Traits, Chesterfield, MO USA
[3] Cluster Cient & Tecnol BioMimic, Red Manejo Biorrac Plagas & Vectores, Inst Ecol AC INECOL, Antigua Carretera Coatepec 351, Xalapa, Veracruz, Mexico
[4] Univ Autonoma Benito Juarez Oaxaca, Fac Med & Cirugia, Exhda Aguilera S-N, Oaxaca 68020, Oaxaca, Mexico
[5] Univ Autonoma Guadalajara, Ave Patria 1201,Col Lomas Valle, Zapopan 45129, Jalisco, Mexico
[6] Consejo Nacl Invest Cient & Tecn, Lab Invest Ecoetol Moscas Fruta & sus Enemigos Na, PROIMI Biotecnol, Ave Belgrano & Pasaje Caseros s-n, RA-4000 San Miguel De Tucuman, Argentina
[7] Coll Wooster, Dept Biol, Coll Mall 931, Wooster, OH 44691 USA
关键词
accessory glands; Anastrepha ludens; ejaculatory bulb; seminal fluid proteins; Tephritidae; testes; ANASTREPHA-LUDENS DIPTERA; SEMINAL FLUID PROTEINS; PEPTIDE IDENTIFICATION; OLFACTORY BEHAVIOR; MATING SUCCESS; DROSOPHILA; DYNAMICS; TEPHRITIDAE; SCRIBBLE; GENES;
D O I
10.1098/rspb.2021.2806
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Seminal fluid proteins (Sfps) modify female phenotypes and have wide-ranging evolutionary implications on fitness in many insects. However, in the Mexican fruit fly, Anastrepha ludens, a highly destructive agricultural pest, the functions of Sfps are still largely unknown. To gain insights into female phenotypes regulated by Sfps, we used nano-liquid chromatography mass spectrometry to conduct a proteomic analysis of the soluble proteins from reproductive organs of A. ludens. The proteins predicted to be transferred from males to females during copulation were 100 proteins from the accessory glands, 69 from the testes and 20 from the ejaculatory bulb, resulting in 141 unique proteins after accounting for redundancies from multiple tissues. These 141 included orthologues to Drosophila melanogaster proteins involved mainly in oogenesis, spermatogenesis, immune response, lifespan and fecundity. In particular, we found one protein associated with female olfactory response to repellent stimuli (Scribble), and two related to memory formation (aPKC and Shibire). Together, these results raise the possibility that A. ludens Sfps could play a role in regulating female olfactory responses and memory formation and could be indicative of novel evolutionary functions in this important agricultural pest.
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收藏
页数:9
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