Circular dichroism (CD) spectroscopy of the assembly and disassembly of SNAREs: The proteins involved in membrane fusion in cells

被引：16

作者：

Cook, Jeremy D. ^{[2
]}

Cho, Won Jin ^{[1
]}

Stemmler, Timothy L. ^{[2
]}

Jena, Bhanu P. ^{[1
]}

机构：

[1] Wayne State Univ, Sch Med, Dept Physiol, Detroit, MI 48201 USA

[2] Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI 48201 USA

来源：

CHEMICAL PHYSICS LETTERS | 2008年 / 462卷 / 1-3期

关键词：

D O I：

10.1016/j.cplett.2008.07.043

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this study, we report for the first time that both t-SNAREs and v-SNARE and their complexes in buffered suspension, exhibit defined peaks at CD signals of 208 and 222 nm wavelengths, consistent with a higher degree of helical secondary structure. Surprisingly, when incorporated in lipid membrane, both SNAREs and their complexes exhibit reduced folding. In presence of NSF-ATP, the SNARE complex disassembles, as reflected from the CD signals demonstrating elimination of alpha-helices within the structure. Published by Elsevier B. V.

引用

页码：6 / 9

页数：4

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