Ruthenium(II) Polypyridyl Complexes: Synthesis and Studies of DNA Binding, Photocleavage, Cytotoxicity, Apoptosis, Cellular Uptake, and Antioxidant Activity

被引：18

作者：

Liu, Yun-Jun ^{[1
]}

Liang, Zhen-Hua ^{[1
]}

Li, Zheng-Zheng ^{[1
]}

Yao, Jun-Hua ^{[2
]}

Huang, Hong-Liang ^{[3
]}

机构：

[1] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Instrumentat Anal & Res Ctr, Guangzhou 510275, Guangdong, Peoples R China

[3] Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China

来源：

DNA AND CELL BIOLOGY | 2011年 / 30卷 / 10期

关键词：

CALF THYMUS DNA; MOLECULAR LIGHT SWITCH; DOUBLE-HELICAL DNA; DEOXYRIBONUCLEIC-ACID; LUMINESCENCE; CLEAVAGE; DRUGS; CELLS;

D O I：

10.1089/dna.2010.1170

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two ruthenium (II) complexes [Ru(dmb)(2)(APIP)](ClO(4))(2) (APIP = 2(2-aminophenyl)imidazo[4,5-f][1,10]phenanthroline, dmb = 4,4'-dimethyl-2,2'-bipyridine; 1) and [Ru(dmb)(2)(HAPIP)](ClO(4))(2) (HAPIP = 2-(2-hydroxyl-4-aminophenyl) imidazo[4,5-f][1,10]phenanthroline; 2) were synthesized and characterized. DNA binding was investigated by electronic absorption titration, luminescence spectra, thermal denaturation, viscosity measurements, and photocleavage. The DNA binding constants for complexes 1 and 2 were 4.20 (+/- 0.14) x 10(4) and 5.45 (+/- 0.15) x 10(4) M(-1). The results suggest that these complexes partially intercalate between the base pairs. The cytotoxicity of complexes 1 and 2 was evaluated by MTT assay. Cellular uptake was observed under fluorescence microscopy; complexes 1 and 2 can enter into the cytoplasm and accumulate in the nuclei. Apoptosis and the antioxidant activity against hydroxyl radicals ((center dot)OH) were also explored.

引用

页码：829 / 838

页数：10

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