Trans-Differentiation of Human Dental Pulp Stem Cells Into Cholinergic-Like Neurons Via Nerve Growth Factor

被引：12

作者：

Darabi, Shahram ^{[1
]}

Tiraihi, Taki ^{[2
]}

Bojnordi, Maryam Nazm ^{[3
,4
]}

Hamidabadi, Hatef Ghasemi ^{[3
,4
]}

Rezaei, Nourollah ^{[3
,4
]}

Zahiri, Maria ^{[5
,6
]}

Alizadeh, Rafieh ^{[7
]}

机构：

[1] Qazvin Univ Med Sci, Cellular & Mol Res Ctr, Qazvin, Iran

[2] Tarbiat Modares Univ, Fac Med Sci, Dept Anat Sci, Tehran, Iran

[3] Mazandaran Univ Med Sci, Fac Med, Dept Anat & Cell Biol, Sari, Iran

[4] Mazandaran Univ Med Sci, Fac Med, Immunogenet Res Ctr, Dept Anat & Cell Biol, Sari, Iran

[5] Bushehr Univ Med Sci, Persian Gulf Biomed Sci Res Inst, Persian Gulf Marine Biotechnol Res Ctr, Bushehr, Iran

[6] Bushehr Univ Med Sci, Sch Med Sci, Dept Anat Sci, Bushehr, Iran

[7] Iran Univ Med Sci, Haarat Rasoul Akrcun Hosp, ENT & Head & Neck Res Ctr & Dept, Tehran, Iran

来源：

BASIC AND CLINICAL NEUROSCIENCE | 2019年 / 10卷 / 06期

关键词：

Dental pulp; Stem cells; Cholinergic neurons; Differentiation; Nerve growth factor; IN-VITRO DIFFERENTIATION; MESENCHYMAL STROMAL CELLS; SPINAL-CORD; DOPAMINERGIC-NEURONS; RETINOIC ACID; TISSUE-REPAIR; TRANSDIFFERENTIATION; EXPRESSION; THERAPY; NEUROGENESIS;

D O I：

10.32598/bcn.10.6.609

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Introduction: Cell therapy has been widely considered as a therapeutic approach for neurodegenerative diseases and nervous system damage. Cholinergic neurons as one of the most important neurons that play a significant role in controlling emotions, mobility, and autonomic systems. In this study, Human Dental Pulp Stem Cells (hDPSCs) were differentiated into the cholinergic neurons by beta-mercaptoethanol in the preinduction phase and also by the nerve growth factor (NGF) in the induction phase. Methods: The hDPSCs were evaluated for CD73, CD31, CD34, and Oct-4. Concentration-time relationships for NGF were assessed by evaluating the viability rate of cells and the immune response to nestin, neurofilament 160, microtubule-associated protein-2, and choline acetyltransferase. Results: The hDPSCs had a negative response to CD34 and CD31. The optimal dose for the NGF was 50 ng/mL seven days after the induction when the highest percentage of expressing markers for the Cholinergic neurons (ChAT) was detected. Conclusion: The results of this study provided a method for producing cholinergic neurons by hDPSCs, which can be used in cytotherapy for degenerative diseases of the nervous system and also spinal cord injury.

引用

页码：609 / 617

页数：9