Development of new DNA markers linked to the Fusarium wilt resistance locus Fom-1 in melon

被引：16
作者：
Tezuka, T. [1 ]
Waki, K. [2 ]
Kuzuya, M. [3 ]
Ishikawa, T. [2 ]
Takatsu, Y. [4 ]
Miyagi, M. [2 ]
机构：
[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Osaka 5998531, Japan
[2] Ibaraki Agr Ctr, Inst Plant Biotechnol, Kasama, Ibaraki 3190292, Japan
[3] Agr & Forestry Off Western Ibaraki, Chikusei, Ibaraki 3080841, Japan
[4] Ibaraki Agr Ctr, Ibaraki Agr Acad, Higashi, Ibaraki 3113116, Japan
来源：
PLANT BREEDING | 2011年 / 130卷 / 02期
关键词：
CAPS marker; Cucumis melo; Fom-1; Fusarium wilt resistance; linkage analysis; recombinant inbred line; F-SP-MELONIS; CONFERRING RESISTANCE; BAC LIBRARY; RACE; GENE; IDENTIFICATION; INHERITANCE; MUSKMELON; CONSTRUCTION; MAP;
D O I：
10.1111/j.1439-0523.2010.01800.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
P>Use of resistance genes would be the most effective way to address Fusarium wilt caused by Fusarium oxysporum f.sp. melonis in melon. In this study, based on the BAC 31O16 sequence which contains the cluster of melon resistance gene homologue, we developed four new DNA markers linked to Fom-1, a dominant resistance gene against F. oxysporum f.sp. melonis races 0 and 2. Linkage analysis using 125 F-2 plants derived from the cross between P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1) indicated that Fom-1 was located between CAPS markers C-MRGH12 and 62-CAPS at a distance of 0.4 and 1.2 cM, respectively. Map position of Fom-1 was confirmed using another mapping population consisting of 104 recombinant inbred lines derived from the cross P11 x MR-1. The analysis of 43 fixed lines and 27 commercial F-1 hybrids showed that the developed DNA markers linked to Fom-1 correctly predicted the genotype in relation to the variety, suggesting that the region around the Fom-1 locus is very polymorphic or there is no linkage disequilibrium between the locus and the marker loci.
引用
收藏
页码：261 / 267
页数：7
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