Monitoring CD4 in Whole Blood with an Opto-fluidic Detector Based on Spatially Modulated Fluorescence Emission

被引：23

作者：

Kiesel, Peter ^{[1
]}

Beck, Markus ^{[1
]}

Johnson, Noble ^{[1
]}

机构：

[1] Xerox Corp, Palo Alto Res Ctr, Elect Mat & Devices Lab, Palo Alto, CA 94304 USA

来源：

CYTOMETRY PART A | 2011年 / 79A卷 / 04期

关键词：

flow cytometer; optofluidics; microfluidics; optical detection; CD4; AIDS diagnostics; point of care testing; IMAGE CYTOMETER; FLOW; ENUMERATION; SYSTEM; TRENDS;

D O I：

10.1002/cyto.a.21042

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a new optical detection technique, termed "spatially modulated fluorescence emission," that delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use. The detection technique is demonstrated with measurements of absolute CD4+ and percentage CD4 counts in human blood. Benchmarking against a commonly used commercial instrument for this test yields excellent agreement for both absolute CD4 and percentage CD4. (C) 2011 International Society for Advancement of Cytometry

引用

页码：317 / 324

页数：8

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