DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES

被引：0

作者：

Petr, Marek ^{[1
]}

Bazantova, Pavla ^{[1
,2
]}

Adamik, Matej ^{[1
]}

Kejnovska, Iva ^{[1
]}

Dvorakova, Zuzana ^{[1
]}

Vorlickova, Michaela ^{[1
]}

Pecinka, Petr ^{[1
,2
]}

Brazdova, Marie ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, Vvi, CS-61265 Brno, Czech Republic

[2] Univ Ostrava, Fac Sci, CZ-70103 Ostrava, Czech Republic

来源：

CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS | 2014年

关键词：

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work we performed DNA polymerase stop assays while using template DNA derived from promoter regions of VEGF and c-Myc proto-oncogenes under variety of experimental conditions. Partial stopping of DNA synthesis along the template strand was observed in the presence of K+ ions due to formation of stable G-quadruplex structures. In contrast, Na+ ions alone were unable to stabilize G-quadruplexes to stop the reaction at their site. These data suggest that K+ and Na+ ions, which are both known to stabilize G-quadruplexes, do this in different manner or extent.

引用

页码：349 / 353

页数：5

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