The microbiome affects liver sphingolipids and plasma fatty acids in a murine model of the Western diet based on soybean oil

被引:6
作者
Di Rienzi, Sara C. [1 ]
Johnson, Elizabeth L. [2 ]
Waters, Jillian L. [3 ]
Kennedy, Elizabeth A. [1 ]
Jacobson, Juliet [1 ]
Lawrence, Peter [2 ]
Wang, Dong Hao [2 ]
Worgall, Tilla S. [4 ]
Brenna, J. Thomas [2 ,5 ]
Ley, Ruth E. [1 ,3 ]
机构
[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY USA
[2] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA
[3] Max Planck Inst Dev Biol, Dept Microbiome Sci, D-72076 Tubingen, Germany
[4] Columbia Univ, Dept Pathol & Cell Biol & Pediat, Med Ctr, New York, NY USA
[5] Univ Texas Austin, Dell Med Sch, Dell Pediat Res Inst, Austin, TX 78712 USA
关键词
germfree; adiposity; linoleic acid; ceramide; sphingomyelin; sphinganine; GUT MICROBIOTA; INSULIN-RESISTANCE; INDUCED OBESITY; INFLAMMATION; METABOLISM; BIOMARKERS; CROSSTALK; LIPIDS; MICE;
D O I
10.1016/j.jnutbio.2021.108808
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Studies in mice using germfree animals as controls for microbial colonization have shown that the gut microbiome mediates diet-induced obesity. Such studies use diets rich in saturated fat, however, Western diets in the United States America are enriched in soybean oil, composed of unsaturated fatty acids, either linoleic or oleic acid. Here, we addressed whether the microbiome is a variable in fat metabolism in mice on a soybean oil diet. We used conventionally-raised, low-germ, and germfree mice fed for 10 weeks diets either high or low in high-linoleic-acid soybean oil as the sole source of fat. Conventional and germfree mice gained relative fat weight and all mice consumed more calories on the high fat vs. low fat soybean oil diet. Plasma fatty acid levels were generally dependent on diet, with microbial colonization status affecting iso-C18:0, C20:3n-6, C14:0, and C15:0 levels. Colonization status, but not diet, impacted levels of liver sphingolipids including ceramides, sphingomyelins, and sphinganine. Our results confirm that absorbed fatty acids are mainly a reflection of the diet and that microbial colonization influences liver sphingolipid pools regardless of diet. (c) 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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页数:13
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