A differential adenosine triphosphate test method for differentiating between bacterial and fungal contamination in water-miscible metalworking fluids

被引：3

作者：

Passman, Frederick J. ^{[1
]}

Kuenzi, Peter ^{[2
]}

机构：

[1] Biodeteriorat Control Associates Inc, Princeton, NJ 08543 USA

[2] Blaser Swisslube, CH-3415 Hasle Ruegsau, Switzerland

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2015年 / 99卷

关键词：

Adenosine triphosphate; ATP; Bacteria; Bioburden; Fungi; Condition monitoring; Metalworking fluid; Microbiological testing; MWF; Rapid methods;

D O I：

10.1016/j.ibiod.2015.01.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new variation of a method for quantifying cellular adenosine triphosphate (cATP) is described. The variation permits differentiation between cATP from bacteria and cATP from fungi. This paper describes the process by which the new differential cATP method was developed and validate in both laboratory and field tests. Laboratory tests using bacteria, yeasts and filamentous fungi demonstrated that cATP observed in >= 5 mu m and <5 mu m size-fractions, respectively were consistently within +/- 10% of predicted concentrations. The field evaluation during which four participating metalworking fluid (MWF) compounders collectively tested >250 used MWF samples, confirmed that the differential protocol reliably detected fungal contamination when it was present. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：129 / 137

页数：9

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