Pulsed ELDOR in spin-labeled polypeptides

被引：56
作者：
Milov, AD
Maryasov, AG [1 ]
Tsvetkov, YD
Raap, J
机构：
[1] Russian Acad Sci, Inst Chem Kinet & Combust, Novosibirsk 630090, Russia
[2] Leiden Univ, Leiden Inst Chem, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands
来源：
CHEMICAL PHYSICS LETTERS | 1999年 / 303卷 / 1-2期
基金：
俄罗斯基础研究基金会;
关键词：
D O I：
10.1016/S0009-2614(99)00220-1
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The pulsed electron-electron double-resonance (PELDOR) technique was applied to obtain information about the structure of the synthetic polypeptide-biradical in a frozen glassy solution. From the concentration dependence of the PELDOR signal, the effects of intermolecular and intramolecular interactions were separated. It was found that the intramolecular dipole-dipole interactions in the biradical peptide led to the modulation effects in the PELDOR signal decay. This may be attributed to the existence of a conformational population having a distance between the two unpaired electrons of similar to 20 Angstrom with a distribution of (similar to 2 Angstrom). Its fraction is estimated as about 25%. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：135 / 143
页数：9
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