Pulsed ELDOR in spin-labeled polypeptides

被引：56

作者：

Milov, AD

Maryasov, AG ^{[1
]}

Tsvetkov, YD

Raap, J

机构：

[1] Russian Acad Sci, Inst Chem Kinet & Combust, Novosibirsk 630090, Russia

[2] Leiden Univ, Leiden Inst Chem, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands

来源：

CHEMICAL PHYSICS LETTERS | 1999年 / 303卷 / 1-2期

基金：

俄罗斯基础研究基金会;

关键词：

D O I：

10.1016/S0009-2614(99)00220-1

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The pulsed electron-electron double-resonance (PELDOR) technique was applied to obtain information about the structure of the synthetic polypeptide-biradical in a frozen glassy solution. From the concentration dependence of the PELDOR signal, the effects of intermolecular and intramolecular interactions were separated. It was found that the intramolecular dipole-dipole interactions in the biradical peptide led to the modulation effects in the PELDOR signal decay. This may be attributed to the existence of a conformational population having a distance between the two unpaired electrons of similar to 20 Angstrom with a distribution of (similar to 2 Angstrom). Its fraction is estimated as about 25%. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：135 / 143

页数：9