Optimizing the baculovirus expression vector system

被引：53

作者：

Hitchman, Richard B. ^{[1
]}

Locanto, Elisabetta ^{[2
]}

Possee, Robert D. ^{[3
]}

King, Linda A. ^{[2
]}

机构：

[1] Oxford Express Technol Ltd, Oxford OX3 0BP, England

[2] Oxford Brookes Univ, Sch Life Sci, Oxford OX3 0BP, England

[3] Ctr Ecol & Hydrol, Wallingford OX10 8BB, Oxon, England

来源：

METHODS | 2011年 / 55卷 / 01期

基金：

英国自然环境研究理事会;

关键词：

Baculovirus; Optimization; flashBAC; High throughput; Protein expression; NUCLEAR POLYHEDROSIS-VIRUS; AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS; INSECT-CELL CULTURES; PROTEIN-PRODUCTION; ENDOPLASMIC-RETICULUM; P10; GENE; RECOMBINANT PROTEINS; ENHANCED EXPRESSION; NUCLEOTIDE-SEQUENCE;

D O I：

10.1016/j.ymeth.2011.06.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Baculoviruses have a unique bi-phasic life cycle and powerful promoters, which greatly facilitates their use for recombinant protein expression in insect cells. We have developed an expression system that utilizes homologous recombination in insect cells between a transfer plasmid containing a gene to be expressed and a replication-deficient virus (bacmid). Only recombinant virus can replicate facilitating the rapid production of multiple recombinant viruses using robotic liquid handlers. The bacmid has also been genetically optimized for improved protein expression and stability. We describe the application of this system for high level production of recombinant proteins. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：52 / 57

页数：6

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