High capacity substrates as a platform for a DNA probe array genotyping assay

被引：13

作者：

Fidanza, J ^{[1
]}

Glazer, M

Mutnick, D

McGall, G

Frank, C

机构：

[1] Affymetrix Inc, Santa Clara, CA USA

[2] Stanford Univ, Stanford, CA 94305 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Colloidal silica particles were deposited on a glass substrate to produce high-capacity porous supports for high-density DNA probe arrays. Porous surfaces were used to increase the addressable surface area and number of probes available for hybridization. Surfaces derived from 70-100 nm size particles deposited in films from 0.15 to 2 microns thick exhibited excellent performance in light-directed oligonucleotide synthesis. Evaluation of these substrates in a genotyping assay is reported.

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页码：533 / 538

页数：6

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