Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

被引：6

作者：

Chen Shuai ^{[1
]}

Liu Guan-shan ^{[1
]}

Wang Yuan-ying ^{[1
]}

Sun Yu-he ^{[1
]}

Chen Jia ^{[2
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Genet Improvement & Biotechnol, Tobacco Res Inst, Qingdao 166101, Peoples R China

[2] China Agr Univ, State Key Lab Plant Physiol & Biochem, Coll Biol Sci, Beijing 100193, Peoples R China

来源：

AGRICULTURAL SCIENCES IN CHINA | 2011年 / 10卷 / 12期

关键词：

abiotic stress; CDPK; Nicotiana tabacum; RACE; real-time qRT-PCR; SIGNALING PATHWAYS; OVER-EXPRESSION; VICIA-FABA; RICE; FAMILY; COLD; EVOLUTIONARY; SPECIFICITY; TOLERANCE; CONFERS;

D O I：

10.1016/S1671-2927(11)60185-5

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.

引用

页码：1851 / 1860

页数：10

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