High-sensitivity urea sensor based on the composite film of electroinactive polypyrrole with polyion complex

被引:48
|
作者
Osaka, T
Komaba, S
Seyama, M
Tanabe, K
机构
[1] Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, 169, Shinjuku-ku
关键词
biosensor; urea sensor; polypyrrole; polyion complex; electropolymerization;
D O I
10.1016/S0925-4005(97)80114-1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A high-sensitivity urea sensor consisting of the composite film of electropolymerized electroinactive polypyrrole (PPy) with the polyion complex containing urease (Urs) was fabricated. This sensor showed potential response to urea within 20 s by detection of pH change during enzymatic reaction with a pH-sensitive electroinactive PPy. The urea response became higher with the slope of 110 mV decade(-1). This high sensitivity was attributed to the effective immobilization of the large number of Urs by electropolymerization on the electrode pre-coated with polyion complex.
引用
收藏
页码:463 / 469
页数:7
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