Streptomyces cerasinus sp nov., isolated from soil in Thailand

A novel actinomycete, strain SR3-134(T), belonging to the genus Streptomyces, was isolated from soil collected from the Sakaerat Environmental Research Station, Thailand Institute of Scientific and Technological Research, Nakhon Ratchasima Province, Thailand. The taxonomic position of the strain was characterized by using a polyphasic approach. LL-Diaminopimelic acid, glucose, mannose and ribose were detected in its whole-cell hydrolysates. The N-acyl type of muramic acid was acetyl. The menaquinones were MK-9(H-g), MK-9(H-6), MK-9(H-4) and MK-9(H-2). The predominant cellular fatty acids were anteiso-C-15:0, iso-C-16 : 0, C-16 : 0, iso-C-15 : 0, anteiso-C-17 : 0 and iso-C-14 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. BLAST analysis of the almost-complete 16S rRNA gene showed 98.7% sequence similarities to Streptomyces lanatus JCM 4588(T) and Streptomyces psammoticus JCM 4434(T). The DNA G+C content was 71.4 mol%. Strain SR3-134(T) showed low DNA-DNA relatedness (12.9 +/- 4.044.1 +/- 1.0 %) to S. lanatus JCM 4588(T) and S. psammoticus JCM 4434(T). The new strain could also be distinguished from its closely related strains by differences in their phenotypic characteristics. The results of taxonomic analysis suggested that strain SR3134(T) represented a novel species of the genus Streptomyces for which the name Streptomyces cerasinus sp. nov. is proposed. The type strain is SR3-134(T) (= TISTR 2494(T) = KCTC 39910(T)).