An In-House Assay Is Superior to Sepsityper for Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification of Yeast Species in Blood Cultures

被引：29

作者：

Bidart, Marie ^{[1
]}

Bonnet, Isabelle ^{[2
]}

Hennebique, Aurelie ^{[2
]}

Kherraf, Zine Eddine ^{[2
]}

Pelloux, Herve ^{[2
]}

Berger, Francois ^{[1
]}

Cornet, Muriel ^{[2
,3
]}

Bailly, Sebastien ^{[2
,4
,5
]}

Maubon, Daniele ^{[2
,3
]}

机构：

[1] CHU Grenoble, UF Clinatec, Pole Rech, F-38043 Grenoble, France

[2] CHU Grenoble, Lab Parasitol Mycol, Inst Biol & Pathol, F-38043 Grenoble, France

[3] Univ Grenoble Alpes, UMR CNRS UJF 5525, Lab TIMC IMAG TheREx, Grenoble, France

[4] Grenoble Alpes Univ, U823, Grenoble, France

[5] Sorbonne Paris Cite Univ, Inserm Paris Diderot, UMR IAME Team DeSCID 5 1137, Paris, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 05期

关键词：

BACTERIA; BOTTLES; SYSTEM; BROTHS; KIT;

D O I：

10.1128/JCM.03600-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification).

引用

页码：1761 / 1764

页数：4

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