共 30 条
Characterization and neural differentiation of mouse embryonic and induced pluripotent stem cells on cadherin-based substrata
被引:51
作者:
Haque, Amranul
[2
]
Yue, Xiao-Shan
[3
]
Motazedian, Ali
[2
]
Tagawa, Yoh-ichi
[2
]
Akaike, Toshihiro
[1
]
机构:
[1] Tokyo Inst Technol, Frontier Res Ctr, Midori Ku, Yokohama, Kanagawa 2268501, Japan
[2] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
[3] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
关键词:
ES/iPS cells;
Monolayer differentiation;
Cadherins;
Extracellular matrix (ECM);
Neuroectoderm;
ES CELLS;
ADHESION;
PRECURSORS;
GENERATION;
D O I:
10.1016/j.biomaterials.2012.04.003
中图分类号:
R318 [生物医学工程];
学科分类号:
0831 ;
摘要:
A suitable culture condition using advanced biomaterials has the potential to improve stem cell differentiation into selective lineages. In this study, we evaluated the effects of recombinant extracellular matrix (ECM) components on the mouse embryonic stem (mES) and induced pluripotent stem (miPS) cells' self-renewal and differentiation into neural progenitors, comparing conventional culture substrata. The recombinant ECMs were established by immobilizing two chimera proteins of cadherin molecules, E-cadherin-Fc and N-cadherin-Fc, either alone or in combination. We report that the completely homogeneous population of mES and miPS cells could be maintained on E-cadherin-based substrata under feeder- and serum-free culture conditions to initiate neural differentiation. Using defined monolayer differentiation conditions on E-cadherin and N-cadherin (E-/N-cad-Fc) hybrid substratum, we routinely obtained highly homogeneous population of primitive ectoderm and neural progenitor cells. Moreover, the differentiated cells with higher expression of beta III-tubulin, Pax6, and tyrosine hydroxylase (TH) in absence of GFAP (a glial cell marker) expression suggesting the presence of a lineage restricted to neural cells. Our improved culture method should provide a homogeneous microenvironment for differentiation and obviate the need for protocols based on stromal feeders or embryoid bodies. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5094 / 5106
页数:13
相关论文