Indirect Spectrophotometric Estimation of Ciprofloxacin Hydrochloride in Pharmaceuticals using N-Bromosuccinimide and Methylene Blue Dye

The present study aims to develop a swift , simple, accurate and sensitive spectrophotometric method for determination of Ciprofloxacin Hydrochloride (CPFX) in pure and pharmaceutical formulations. The suggested method depend on oxidation of CPFX with known excess amount of N-bromosuccinimide (NBS) in acidic medium and after reaction is insured to be complete , the surplus NBS is estimated by de colorization of methylene blue dye and measuring the absorbance of surplus dye at 665 nm. A linear calibration curve was obtained over the concentration range 0.5 - 4.5 mu g.ml(-1) with correlation coefficient of 0.9993. The molar absorptivity and sandell's sensitivity index values were determined to be 8.6 x10(4) L.mol(-1).cm(-1) and 0.004276 mu g.cm(-2), respectively .The limit of detection (LOD) and quantification (LOQ) were calculated to be 0.0033 and 0.0111 mu g.ml(-1), respectively . The proposed method has been successfully applied to the determination of CIPH in available dosage form, the validity proposed method was confirmed by recovery study via standard addition technique .