Liquid-Phase Electron Microscopy for Soft Matter Science and Biology

被引：95
|
作者：
Wu, Hanglong [1 ]
Friedrich, Heiner [1 ,2 ]
Patterson, Joseph P. [3 ]
Sommerdijk, Nico A. J. M. [4 ]
de Jonge, Niels [5 ,6 ]
机构：
[1] Eindhoven Univ Technol, Dept Chem Engn & Chem, NL-5600 MB Eindhoven, Netherlands
[2] Eindhoven Univ Technol, Inst Complex Mol Syst, NL-5600 MB Eindhoven, Netherlands
[3] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA
[4] Radboud Univ Nijmegen, Med Ctr, Dept Biochem, NL-6500 HB Nijmegen, Netherlands
[5] INM Leibniz Inst New Mat, D-66123 Saarbrucken, Germany
[6] Saarland Univ, Dept Phys, D-66123 Saarbrucken, Germany
来源：
ADVANCED MATERIALS | 2020年 / 32卷 / 25期
关键词：
beam-sample interactions; dynamic processes; in situ electron microscopy; synthetic materials; IN-SITU; RADIATION-DAMAGE; GOLD NANOPARTICLES; GROWTH-KINETICS; WHOLE CELLS; WET-STEM; RESOLUTION; GRAPHENE; NANOSCALE; CONTRAST;
D O I：
10.1002/adma.202001582
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Innovations in liquid-phase electron microscopy (LP-EM) have made it possible to perform experiments at the optimized conditions needed to examine soft matter. The main obstacle is conducting experiments in such a way that electron beam radiation can be used to obtain answers for scientific questions without changing the structure and (bio)chemical processes in the sample due to the influence of the radiation. By overcoming these experimental difficulties at least partially, LP-EM has evolved into a new microscopy method with nanometer spatial resolution and sub-second temporal resolution for analysis of soft matter in materials science and biology. Both experimental design and applications of LP-EM for soft matter materials science and biological research are reviewed, and a perspective of possible future directions is given.
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页数：21
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