The local structures of Cu(II) and Zn(II) complexes of hyaluronate

被引：44

作者：

Nagy, L

Yamashita, S

Yamaguchi, T

Sipos, P

Wakita, H

Nomura, M

机构：

[1] Attila Jozsef Univ, Dept Inorgan & Analyt Chem, H-6701 Szeged, Hungary

[2] Fukuoka Univ, Fac Sci, Dept Chem, Jonan Ku, Fukuoka 81401, Japan

[3] Natl Lab High Energy Phys, Photon Factory, Oho, Ibaraki 305, Japan

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1998年 / 72卷 / 1-2期

基金：

匈牙利科学研究基金会;

关键词：

copper(II); zinc(II); hyaluronate; complex; EXAFS; local structure;

D O I：

10.1016/S0162-0134(98)10061-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cu(II) and Zn(II) complexes of hyaluronate (Hy) were prepared. The local structures and binding sites were determined by Cu and Zn K-EXAFS and XANES measurements in the solid state. Both metal ions were found to be coordinated to Hy via carboxylate and oxygen atoms and probably O-1. The NH group of the acetamido moiety does not take part in the complexation. Analysis of the EXAFS spectra revealed that the oxygen coordination geometry around the Cu(II) ion (distorted octahedron) and the Cu-O bond length (r(eq) = 200 pm) in the Cu(II)-Hy complex are not very different from those in the hexaaqua Cu(II) ion (195 pm). The Zn(II)-Hy complex is tetra coordinated in a tetrahedral structure, with an average Zn-O bond length of 202 pm. The nonbonding Cu ... C and Zn ... C distances were determined to be 315 and 299 pm, respectively. These values and the large Debye-Waller factors indicate the macrochelate coordination mode of the ligand. (C) 1998 Published by Elsevier Science Inc. All rights reserved.

引用

页码：49 / 55

页数：7

共 32 条

[1] Transition-metal saccharide chemistry and biology: Saccharide complexes of Cu(II) and their effect on in vivo metallothionein synthesis in mice [J].