HrpG and HrpV proteins from the Type III secretion system of Erwinia amylovora form a stable heterodimer

被引：10

作者：

Gazi, Anastasia D. ^{[1
,2
]}

Charova, Spyridoula ^{[1
]}

Aivaliotis, Michalis ^{[1
]}

Panopoulos, Nicholas J. ^{[2
,3
]}

Kokkinidis, Michael ^{[1
,2
]}

机构：

[1] Fdn Res & Technol Hellas IMBB FORTH, Inst Mol Biol & Biotechnol, GR-70013 Iraklion, Crete, Greece

[2] Univ Crete, Dept Biol, Iraklion 71409, Crete, Greece

[3] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2015年 / 362卷 / 01期

关键词：

small-angle X-ray scattering (SAXS); bicistronic expression vectors; hypersensitive response and pathogenicity; Pseudomonas syringae; Erwinia amylovora; PSEUDOMONAS-SYRINGAE STRAINS; STRUCTURE PREDICTION; NEGATIVE REGULATION; SOLUTION SCATTERING; PATHOGENICITY; RESOLUTION; BINDING; GENES;

D O I：

10.1093/femsle/fnu011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacterial type III secretion systems (T3SSs) are specialized multicomponent nanomachines that mediate the transport of proteins either to extracellular locations or directly into eukaryotic host cell cytoplasm. Erwinia amylovora, the main agent of rosaceous plants fireblight disease, employs an Hrp/Hrc1 T3SS to accomplish its pathogenesis. The regulatory network that controls the activation of this T3SS is largely unknown in E. amylovora. However, in Pseudomonas syringae pathovars, the HrpG/HrpV complex has been shown to directly regulate the activity of transcription factor HrpS and consequently the upregulation of the Hrp/Hrc1 T3SS related genes. In this work, we report the successful recombinant production and purification of a stable E. amylovora HrpG/HrpV complex, using pPROpET, a bicistronic expression vector. Furthermore, we present the first solution structure of this complex based on small-angle X-ray scattering data.

引用

页数：8

共 25 条

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