Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp strain

被引：31
作者：
Delattre, C [1 ]
Michaud, P [1 ]
Lion, JM [1 ]
Courtois, B [1 ]
Courtois, J [1 ]
机构：
[1] UPJV, IUT GB, Lab Glucides, EPMV,CNRS,FRE 2779, F-80025 Amiens, France
来源：
JOURNAL OF BIOTECHNOLOGY | 2005年 / 118卷 / 04期
关键词：
glucuronan; oligosaccharides; polysaccharide lyases;
D O I：
10.1016/j.jbiotec.2005.04.014
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids beta-(1,4) linked and variably C-2 and/or C-3 O-acetylated. To obtain beta-Delta-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using H-1 NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：448 / 457
页数：10
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