Identification of a Nosema bombycis (Microsporidia) spore wall protein corresponding to spore phagocytosis

被引：37

作者：

Cai, Shunfeng ^{[1
]}

Lu, Xingmeng ^{[1
]}

Qiu, Haihong ^{[1
]}

Li, Mingqian ^{[1
]}

Feng, Zhenzhen ^{[1
]}

机构：

[1] Zhejiang Univ, Lab Invertebrate Pathol, Hangzhou 310029, Zhejiang, Peoples R China

来源：

PARASITOLOGY | 2011年 / 138卷 / 09期

关键词：

Microsporidia; Nosema bombycis; 2D-PAGE; spore wall protein; sporoblast; phagocytosis; ENCEPHALITOZOON-CUNICULI MICROSPORIDIA; DEVELOPMENTAL EXPRESSION; SPOROGONIAL STAGES; PROTEOMIC ANALYSIS; LIFE-CYCLE; IN-VITRO; ENDOSPORE; GRADIENT; ENP1;

D O I：

10.1017/S0031182011000801

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Life-cycle stages of the microsporidia Nosema bombycis, the pathogen causing silkworm pebrine, were separated and purified by an improved method of Percoll-gradient centrifugation. Soluble protein fractions of late sporoblasts (spore precursor cells) and mature spores were analysed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Protein spots were recovered from gels and analysed by mass spectrometry. The most abundant differential protein spot was identified by database search to be a hypothetical spore wall protein. Using immunoelectron microscopy, we demonstrated that HSWP5 is localized to the exospore of mature spores and renamed it as spore wall protein 5 (NbSWP5). Further spore phagocytosis assays indicated that NbSWP5 can protect spores from phagocytic uptake by cultured insect cells. This spore wall protein may function both for structural integrity and in modulating host cell invasion.

引用

页码：1102 / 1109

页数：8

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