Application of Real-Time PCR Stool Assay for Helicobacter pylori Detection and Clarithromycin Susceptibility Testing in Brazilian Children

被引：53

作者：

Scaletsky, Isabel C. A. ^{[1
]}

Aranda, Katia R. S. ^{[1
]}

Garcia, Gabriela T. ^{[2
]}

Goncalves, Manoel E. P. ^{[3
]}

Cardoso, Silvia R. ^{[3
]}

Iriya, Kiyoshi ^{[4
]}

Silva, Neusa P. ^{[5
]}

机构：

[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, Dept Pediat, BR-04023062 Sao Paulo, Brazil

[3] Univ Sao Paulo, Fac Med, Inst Crianca, Sao Paulo, Brazil

[4] Univ Sao Paulo, Fac Med, Dept Patol Med, Sao Paulo, Brazil

[5] Univ Fed Sao Paulo, Disciplina Reumatol, BR-04023062 Sao Paulo, Brazil

来源：

HELICOBACTER | 2011年 / 16卷 / 04期

关键词：

Helicobacter pylori; stool PCR; children; INFECTION; PREVALENCE; SPECIMENS; RESISTANCE; VACA; CAGA;

D O I：

10.1111/j.1523-5378.2011.00845.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens. Objective: The aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children. Methods: DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing. Results: The sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance. Conclusion: This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.

引用

页码：311 / 315

页数：5

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