Upregulation of heme oxygenase-1 via PI3K/Akt and Nrf-2 signaling pathways mediates the anti-inflammatory activity of Schisandrin in Porphyromonas gingivalis LPS-stimulated macrophages

被引：71

作者：

Park, Sun Young ^{[2
,3
]}

Park, Da Jung ^{[1
]}

Kim, Young Hun ^{[2
]}

Kim, YoungHee ^{[1
,3
]}

Kim, Sun Gun

Shon, Kwang Jae ^{[4
]}

Choi, Young-Whan ^{[1
]}

Lee, Sang-Joon ^{[5
]}

机构：

[1] Pusan Natl Univ, Dept Hort Biosci, Miryang 627706, South Korea

[2] Pusan Natl Univ, BioIT Fus Technol Res Inst, Pusan 609735, South Korea

[3] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

[4] Pusan Natl Univ, Ctr Res Facil, Pusan 609735, South Korea

[5] Pusan Natl Univ, Dept Microbiol, Pusan 609735, South Korea

来源：

IMMUNOLOGY LETTERS | 2011年 / 139卷 / 1-2期

关键词：

Schisandrin; Porphyromonas gingivalis LPS; Pro-inflammatory cytokine; NF-kappa B; Heme oxygenase-1; Nrf-2; INFLAMMATORY CYTOKINE RELEASE; SCHIZANDRA-CHINENSIS BAILL; MOLECULAR-MECHANISMS; ACTIVATION; EXPRESSION; LIPOPOLYSACCHARIDE; CONSTITUENTS; SURFACTIN; CURCUMIN; LIGNANS;

D O I：

10.1016/j.imlet.2011.05.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The lipopolysaccharide (LPS) of Porphyromonas gingivalis is thought to induce periodontitis. In this study, we isolated Schisandrin from the dried fruits of Schisandra chinensis and examined the anti-inflammatory effect of Schisandrin in macrophages stimulated with LPS from P. gingivalis. First, Schisandrin inhibited LPS-induced pro-inflammatory cytokines, including TNF-alpha, IL-1 beta, and IL-6. And Schisandrin suppressed the nuclear translocation and activity of NF-kappa B and phosphorylation of I kappa B alpha in LPS-stimulated RAW 264.7 cells. Next, the presence of a selective inhibitor of HO-1 (SnPP) and a siRNA specific for HO-1 inhibited Schisandrin-mediated anti-inflammatory activity. Furthermore, Schisandrin induced HO-1 expression of RAW 264.7 cells through Nrf-2, PI3K/Akt, and ERK activation. Therefore, these results suggest that the anti-inflammatory effects of Schisandrin on P. gingivalis LPS-stimulated RAW 264.7 cells may be due to a reduction of NF-kappa B activity and induction of the expression of HO-1, leading to INF-alpha, IL-10, and IL-6 down-regulation. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：93 / 101

页数：9

共 31 条

[1] Heme oxygenase-1 mediates the anti-inflammatory actions of 2′-hydroxychalcone in RAW 264.7 murine macrophages [J].