Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats

被引：43

作者：

Sasaki, Yuichi ^{[1
,2
]}

Sasaki, Masanori ^{[1
,3
,4
]}

Kataoka-Sasaki, Yuko ^{[1
]}

Nakazaki, Masahito ^{[1
]}

Nagahama, Hiroshi ^{[1
]}

Suzuki, Junpei ^{[1
]}

Tateyama, Daiki ^{[1
]}

Oka, Shinichi ^{[1
]}

Namioka, Takahiro ^{[1
]}

Namioka, Ai ^{[1
]}

Onodera, Rie ^{[1
]}

Mikami, Takeshi ^{[5
]}

Wanibuchi, Masahiko ^{[5
]}

Kakizawa, Masafumi ^{[2
]}

Ishiai, Sumio ^{[2
]}

Kocsis, Jeffery D. ^{[3
,4
]}

Honmou, Osamu ^{[1
,3
,4
]}

机构：

[1] Sapporo Med Univ, Sch Med, Res Inst Frontier Med, Dept Neural Regenerat Med, Sapporo, Hokkaido 0608556, Japan

[2] Sapporo Med Univ, Sch Med, Dept Rehabil, Sapporo, Hokkaido, Japan

[3] Yale Univ, Sch Med, Dept Neurol, New Haven, CT 06510 USA

[4] VA Connecticut Healthcare Syst, Ctr Neurosci & Regenerat Res, West Haven, CT 06516 USA

[5] Sapporo Med Univ, Sch Med, Dept Neurosurg, Sapporo, Hokkaido, Japan

来源：

PHYSICAL THERAPY | 2016年 / 96卷 / 11期

关键词：

CEREBRAL-ISCHEMIA MODEL; BONE-MARROW; FUNCTIONAL RECOVERY; STROMAL CELLS; INJURY; PLASTICITY; PROTECTS; ENVIRONMENT; EXERCISE; THERAPY;

D O I：

10.2522/ptj.20150504

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Background. Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia. Objective. The purpose of this study was to investigate whether synergic effects of daily rehabilitation and intravenous infusion of MSCs has therapeutic effects after stroke in rats. Design. This was an experimental study. Methods. A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. Four experimental groups were studied: group 1 (vehicle only, n=10), group 2 (vehicle + exercise, n=10), group 3 (MSCs only, n=10), and group 4 (MSCs + exercise, n=10). Rat MSCs were intravenously infused at 6 hours after MCAO, and the rats received daily rehabilitation with treadmill running exercise for 20 minutes. Lesion size was assessed at 1, 14, and 35 days using magnetic resonance imaging. Functional outcome was assessed using the Limb Placement Test. Results. Both combined therapy and MSC infusion reduced lesion volume, induced synaptogenesis, and elicited functional improvement compared with the groups without MSC infusion, but the effect was greater in the combined therapy group. Limitations. A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans. Conclusions. The data indicate that the combined therapy of daily rehabilitation and intravenous infusion of MSCs improved functional outcome in a rat MCAO model.

引用

页码：1791 / 1798

页数：8

共 39 条

[1] Transplanted Neurally Modified Bone Marrow-Derived Mesenchymal Stem Cells Promote Tissue Protection and Locomotor Recovery in Spinal Cord Injured Rats [J].