Characterization of Prototheca zopfii Associated with Outbreak of Bovine Clinical Mastitis in Herd of Beijing, China

被引：40

作者：

Gao, Jian ^{[1
]}

Zhang, Han-qi ^{[1
]}

He, Jian-zhong ^{[1
]}

He, Yang-hua ^{[2
]}

Li, Shu-mei ^{[3
]}

Hou, Rong-guang ^{[1
]}

Wu, Qiao-xing ^{[1
]}

Gao, Yang ^{[1
]}

Han, Bo ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China

[2] China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

[3] Beijing Lab Anim Res Ctr, Beijing 100012, Peoples R China

来源：

MYCOPATHOLOGIA | 2012年 / 173卷 / 04期

关键词：

Bovine mastitis; Outbreak; Prototheca zopfii; Genotype; 2; In vitro susceptibility test; DAIRY HERDS; ENVIRONMENTAL SOURCES; SHORT-COMMUNICATION; ALGA PROTOTHECA; SUSCEPTIBILITY; IDENTIFICATION; BLASCHKEAE; GENOTYPES; PROPOSAL; STRAINS;

D O I：

10.1007/s11046-011-9510-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Prototheca zopfii (P. zopfii) has become an important cause of bovine mastitis in many countries. In the present study, to better understand the occurrence of one clinical mastitis (CM) outbreak due to P. zopfii, the molecular characterization and resistance patterns of the microalga were described. P. zopfii strains were isolated from 17 of 23 quarters, which suffered CM in the outbreak, and 7 of 46 CM recovered quarters before the outbreak, as well as 2 of 75 environmental samples in the dairy farm. All strains were identified as genotype 2 by genotype-specific PCR analysis. Results of in vitro antimicrobial and antifungal susceptibility tests indicated that these strains were resistant to majority of tested drugs, with the only exception of amphotericin B, nystatin, streptomycin, gentamicin, and amikacin. This is the first report about CM outbreak caused by P. zopfii in China. These data suggest that P. zopfii may represent a serious risk in the studied herd, and this microalga could be an important potential pathogen causing mastitis in dairy herds of Beijing.

引用

页码：275 / 281

页数：7

共 26 条

[1] Identification of Prototheca from bovine mastitis in Denmark [J].

Aalbaek, B ;

Jensen, HE ;

Huda, A .

APMIS, 1998, 106 (04) :483-488

[2]

[Anonymous], 2008, Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts

[3]

Third Informational Supplement. CLSI document M27-S3, P28

[4] Epidemiological and clinical aspects of the first outbreak of bovine mastitis caused by Prototheca zopfii in Goias State, Brazil [J].

Bueno, VFF ;

de Mesquita, AJ ;

Neves, RBS ;

de Souza, MA ;

Ribeiro, AR ;

Nicolau, ES ;

de Oliveira, AN .

MYCOPATHOLOGIA, 2006, 161 (03) :141-145

[5] First large-scale isolation of Prototheca zopfii from milk produced by dairy herds in Italy [J].

Buzzini, P ;

Turchetti, B ;

Facelli, R ;

Baudino, R ;

Cavarero, F ;

Mattalia, L ;

Mosso, P ;

Martini, A .

MYCOPATHOLOGIA, 2004, 158 (04) :427-430

[6] Bovine mastitis due to Prototheca zopfii:: Clinical, epidemiological and pathological aspects in a Brazilian dairy herd [J].

Corbellini, LG ;

Driemeier, D ;

Cruz, C ;

Dias, MM ;

Ferreiro, L .

TROPICAL ANIMAL HEALTH AND PRODUCTION, 2001, 33 (06) :463-470

[7] Epidemiologic study of environmental sources in a Prototheca zopfii outbreak of bovine mastitis [J].

Costa, EO ;

Melville, PA ;

Ribeiro, AR ;

Watanabe, ET ;

Parolari, MCFF .

MYCOPATHOLOGIA, 1997, 137 (01) :33-36

[8] Prototheca sp outbreak of bovine mastitis [J].

Costa, EO ;

Carciofi, AC ;

Melville, PA ;

Prada, MS ;

Schalch, U .

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1996, 43 (06) :321-324

[9]

FRANK N, 1969, AM J VET RES, V30, P1785

[10] Genotyping of bovine Prototheca mastitis isolates from Poland [J].

Jagielski, Tomasz ;

Lassa, Henryka ;

Ahrholdt, Jennifer ;

Malinowski, Edward ;

Roesler, Uwe .

VETERINARY MICROBIOLOGY, 2011, 149 (1-2) :283-287

← 1 2 3 →