RGD-functionalised melanin nanoparticles for intraoperative photoacoustic imaging-guided breast cancer surgery

被引:20
作者
Liu, Jing-Jing [1 ,2 ,3 ]
Wang, Zun [4 ,5 ]
Nie, Li-Ming [6 ,7 ,8 ]
Zhu, Yuan-Yuan [1 ,2 ,3 ]
Li, Ge [1 ,2 ,3 ]
Lin, Lin-Ling [1 ,2 ,3 ]
Chen, Min [3 ,9 ,10 ]
Zhang, Guo-Jun [1 ,2 ,3 ,9 ]
机构
[1] Xiamen Univ, Canc Ctr, Sch Med, Xiangan Hosp Xiamen Univ, 2000 Xiangan Rd East, Xiamen 361101, Fujian, Peoples R China
[2] Xiamen Univ, Dept Breast & Thyroid Surg, Sch Med, Xiangan Hosp Xiamen Univ, 2000 Xiangan Rd East, Xiamen 361101, Fujian, Peoples R China
[3] Xiamen Univ, Xiamen Key Lab Endocrine Related Canc Precis Med, Xiangan Hosp, Xiamen 361101, Fujian, Peoples R China
[4] Shantou Univ, ChangJiang Scholars Lab, Med Coll, Shantou 515041, Guangdong, Peoples R China
[5] Jinan Univ, Shenzhen Baoan Womens & Childrens Hosp, Dept Breast & Thyroid Surg, Shenzhen 518133, Guangdong, Peoples R China
[6] Xiamen Univ, State Key Lab Mol Vaccinol & Mol Diag, Sch Publ Hlth, Xiamen 361102, Fujian, Peoples R China
[7] Xiamen Univ, Ctr Mol Imaging & Translat Med, Sch Publ Hlth, Xiamen 361102, Fujian, Peoples R China
[8] Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Dept Radiol & Opt Imaging Lab, Guangzhou 510080, Guangdong, Peoples R China
[9] Xiamen Univ, Canc Res Ctr, Sch Med, Xiamen 361101, Fujian, Peoples R China
[10] Xiamen Univ, Clin Cent Res Core, Xiangan Hosp, 2000 Xiangan Rd East, Xiamen 361101, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
Melanin; Bioimaging; Photoacoustic imaging; Breast cancer; Tumour detection; Imaging-guided surgery; INTEGRIN ALPHA(V)BETA(3); TOMOGRAPHY; EXPRESSION; MARGIN; AGENTS;
D O I
10.1007/s00259-021-05545-3
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Purpose Obtaining tumour-free margins is critical for avoiding re-excision and reducing local recurrence following breast-conserving surgery; however, it remains challenging. Imaging-guided surgery provides precise detection of residual lesions and assists surgical resection. Herein, we described water-soluble melanin nanoparticles (MNPs) conjugated with cyclic Arg-Gly-Asp (cRGD) peptides for breast cancer photoacoustic imaging (PAI) and surgical navigation. Methods The cRGD-MNPs were synthesised and characterized for morphology, photoacoustic characteristics and stability. Tumour targeting and toxicity of cRGD-MNPs were determined by using either breast cancer cells, MDA-MB-231 tumour-bearing mice or the FVB/N-Tg (MMTV-PyVT) 634Mul/J mice model. PAI was used to locate the tumour and guide surgical resection in MDA-MB-231 tumour-bearing mice. Results The cRGD-MNPs exhibited excellent in vitro and in vivo tumour targeting with low toxicity. Intravenous administration of cRGD-MNPs to MDA-MB-231 tumour-bearing mice showed an approximately 2.1-fold enhancement in photoacoustic (PA) intensity at 2 h, and the ratio of the PA intensity at the tumour site to that in the surrounding normal tissue was 3.2 +/- 0.1, which was higher than that using MNPs (1.7 +/- 0.3). Similarly, the PA signal in the spontaneous breast cancer increased similar to 2.5-fold at 2 h post-injection of cRGD-MNPs in MMTV-PyVT transgenic mice. Preoperative PAI assessed tumour volume and offered three-dimensional (3D) reconstruction images for accurate surgical planning. Surgical resection following real-time PAI showed high consistency with histopathological analysis. Conclusion These results highlight that cRGD-MNP-mediated PAI provide a powerful tool for breast cancer imaging and precise tumour resection. cRGD-MNPs with fine PA properties have great potential for clinical translation.
引用
收藏
页码:847 / 860
页数:14
相关论文
共 43 条
  • [1] Intra-operative ultrasound versus wire-guided localization in the surgical management of non-palpable breast cancers: systematic review and meta-analysis
    Ahmed, M.
    Douek, M.
    [J]. BREAST CANCER RESEARCH AND TREATMENT, 2013, 140 (03) : 435 - 446
  • [2] Patterns of αvβ3 expression in primary and metastatic human breast cancer as shown by 18F-galacto-RGD PET
    Beer, Ambros J.
    Niemeyer, Markus
    Carlsen, Janette
    Sarbia, Mario
    Naehrig, Joerg
    Watzlowik, Petra
    Wester, Hans-Juergen
    Harbeck, Nadia
    Schwaiger, Markus
    [J]. JOURNAL OF NUCLEAR MEDICINE, 2008, 49 (02) : 255 - 259
  • [3] Hemotoxicity of carbon nanotubes
    Bussy, Cyrill
    Methven, Laura
    Kostarelos, Kostas
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2013, 65 (15) : 2127 - 2134
  • [4] Melanin nanoparticles as a promising tool for biomedical applications - a review
    Caldas, Mariana
    Santos, Ana Claudia
    Veiga, Francisco
    Rebelo, Rita
    Reis, Rui L.
    Correlo, Vitor M.
    [J]. ACTA BIOMATERIALIA, 2020, 105 : 26 - 43
  • [5] Carbon nanotubes as photoacoustic molecular imaging agents in living mice
    De La Zerda, Adam
    Zavaleta, Cristina
    Keren, Shay
    Vaithilingam, Srikant
    Bodapati, Sunil
    Liu, Zhuang
    Levi, Jelena
    Smith, Bryan R.
    Ma, Te-Jen
    Oralkan, Omer
    Cheng, Zhen
    Chen, Xiaoyuan
    Dai, Hongjie
    Khuri-Yakub, Butrus T.
    Gambhir, Sanjiv S.
    [J]. NATURE NANOTECHNOLOGY, 2008, 3 (09) : 557 - 562
  • [6] Breast cancer statistics, 2019
    DeSantis, Carol E.
    Ma, Jiemin
    Gaudet, Mia M.
    Newman, Lisa A.
    Miller, Kimberly D.
    Sauer, Ann Goding
    Jemal, Ahmedin
    Siegel, Rebecca L.
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2019, 69 (06) : 438 - 451
  • [7] Effect of radiotherapy after breast-conserving surgery on 10-year recurrence and 15-year breast cancer death: meta-analysis of individual patient data for 10 801 women in 17 randomised trials
    Darby S.
    McGale P.
    Correa C.
    Taylor C.
    Arriagada R.
    Clarke M.
    Cutter D.
    Davies C.
    Ewertz M.
    Godwin J.
    Gray R.
    Pierce L.
    Whelan T.
    Wang Y.
    Peto R.
    Albain K.
    Anderson S.
    Barlow W.
    Bergh J.
    Bliss J.
    Buyse M.
    Cameron D.
    Carrasco E.
    Coates A.
    Collins R.
    Costantino J.
    Cuzick J.
    Davidson N.
    Davies K.
    Delmestri A.
    Di Leo A.
    Dowsett M.
    Elphinstone P.
    Evans V.
    Gelber R.
    Gettins L.
    Geyer C.
    Goldhirsch A.
    Gregory C.
    Hayes D.
    Hill C.
    Ingle J.
    Jakesz R.
    James S.
    Kaufmann M.
    Kerr A.
    MacKinnon E.
    McHugh T.
    Norton L.
    Ohashi Y.
    [J]. LANCET, 2011, 378 (9804) : 1707 - 1716
  • [8] Intraoperative Imprint Cytology and Frozen Section Pathology for Margin Assessment in Breast Conservation Surgery: A Systematic Review
    Esbona, Karla
    Li, Zhanhai
    Wilke, Lee G.
    [J]. ANNALS OF SURGICAL ONCOLOGY, 2012, 19 (10) : 3236 - 3245
  • [9] Transferring Biomarker into Molecular Probe: Melanin Nanoparticle as a Naturally Active Platform for Multimodality Imaging
    Fan, Quli
    Cheng, Kai
    Hu, Xiang
    Ma, Xiaowei
    Zhang, Ruiping
    Yang, Min
    Lu, Xiaomei
    Xing, Lei
    Huang, Wei
    Gambhir, Sanjiv Sam
    Cheng, Zhen
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (43) : 15185 - 15194
  • [10] Photoacoustic Imaging: Contrast Agents and Their Biomedical Applications
    Fu, Qinrui
    Zhu, Rong
    Song, Jibin
    Yang, Huanghao
    Chen, Xiaoyuan
    [J]. ADVANCED MATERIALS, 2019, 31 (06)