Inhibition of basal cell carcinoma cells by cold atmospheric plasma-activated solution and differential gene expression analysis

被引：18
|
作者：
Yang, Xingyu [1 ]
Yang, Chunjun [1 ]
Wang, Liyun [1 ]
Cao, Zhicheng [1 ]
Wang, Yuan [2 ]
Cheng, Cheng [3 ]
Zhao, Guoping [4 ]
Zhao, Ye [5 ]
机构：
[1] Anhui Med Univ, Affiliated Hosp 2, Dept Dermatol, 678 Furong Rd, Hefei 230601, Anhui, Peoples R China
[2] Anhui Med Univ, Dept Orthpaed, Affiliated Hosp 1, Hefei 230022, Anhui, Peoples R China
[3] Chinese Acad Sci, Hefei Inst Phys Sci, Inst Plasma Phys, Hefei 230031, Anhui, Peoples R China
[4] Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, Hefei 230031, Anhui, Peoples R China
[5] Anhui Med Univ, Teaching & Res Sect Nucl Med, Hefei 230032, Anhui, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ONCOLOGY | 2020年 / 56卷 / 05期
基金：
中国国家自然科学基金;
关键词：
CAP; PAS; BCC; ROS; RNA-sequencing; MAPK; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; CANCER-CELLS; APOPTOSIS; SELECTIVITY; PATHWAYS; MEDIA;
D O I：
10.3892/ijo.2020.5009
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Basal cell carcinoma is a common skin tumor. Cold atmospheric plasma (CAP) has attracted increasing attention for its antitumor effects. The aim of the present study was to investigate the effects and related mechanisms of two CAP-activated solutions on the TE354T basal cell carcinoma and HaCat keratinocyte cell lines. Plasma-activated solution (PAS) was prepared by CAP irradiation of DMEM and PBS. TE354T cells were treated with PAS in vitro and the effect on cell viability was evaluated by an MTT assay. The apoptosis rate was detected by Annexin V/propidium iodide staining. Furthermore, western blotting and RNA-sequencing were performed. The present results demonstrated that PAS induced apoptotic signaling in basal cell carcinoma cells, and that this effect was associated with the activation of the MAPK signaling pathway. Therefore, the present study demonstrated that PAS may serve as a novel treatment for basal cell carcinoma.
引用
收藏
页码：1262 / 1273
页数：12
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