Direct Measurement of Cell Wall Stress Stiffening and Turgor Pressure in Live Bacterial Cells

被引：177
|
作者：
Deng, Yi [1 ]
Sun, Mingzhai [2 ]
Shaevitz, Joshua W. [1 ,2 ]
机构：
[1] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA
[2] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA
来源：
PHYSICAL REVIEW LETTERS | 2011年 / 107卷 / 15期
基金：
美国国家科学基金会;
关键词：
ATOMIC-FORCE MICROSCOPY; MECHANICAL-PROPERTIES; BACILLUS-SUBTILIS; THREAD; ELASTICITY; SURFACE; WATER; SHAPE;
D O I：
10.1103/PhysRevLett.107.158101
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
We study intact and bulging Escherichia coli cells using atomic force microscopy to separate the contributions of the cell wall and turgor pressure to the overall cell stiffness. We find strong evidence of power-law stress stiffening in the E. coli cell wall, with an exponent of 1.22 +/- 0.12, such that the wall is significantly stiffer in intact cells (E = 23 +/- 8 MPa and 49 +/- 20 MPa in the axial and circumferential directions) than in unpressurized sacculi. These measurements also indicate that the turgor pressure in living cells E. coli is 29 +/- 3 kPa.
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