Development and Validation of an HPLC-MS/MS Analytical Method for Quantitative Determination of Acetylsalicylic Acid and its Metabolite Salicylic Acid in Human Blood Plasma

被引：1

作者：

Tserkovnaya, K. M. ^{[1
]}

Flisyuk, E., V ^{[1
]}

Shabrov, V. N. ^{[2
]}

机构：

[1] St Petersburg State Chem & Pharmaceut Univ, 14A Prof Popova St, Russia 197376, Russia

[2] LLC Quinta Analyt Yaroslavl, 52G Leningradskii Prosp, Yaroslavl 150045, Russia

来源：

PHARMACEUTICAL CHEMISTRY JOURNAL | 2022年 / 55卷 / 12期

关键词：

acetylsalicylic acid; human blood plasma; HPLC; MS; validation; bioequivalence;

D O I：

10.1007/s11094-022-02588-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

An HPLC-MS/MS analytical method for quantitative determination of acetylsalicylic acid (ASA) as an antithrombotic agent and its metabolite salicylic acid (SA) in human blood plasma was developed and validated. The optimum concentration ranges were 0.020 - 1.200 mu g/mL for ASA and 0.200 - 12.00 mu g/mL for SA. The optimum conditions for sample preparation using liquid-liquid extraction by EtOAc followed by removal of the solvent in a stream of N-2, redissolution of the dry residue (to concentrate the sample and increase the method sensitivity), chromatography, and mass-spectrometric detection were selected. The developed method could be used further for pharmacokinetic and bioequivalence studies.

引用

页码：1401 / 1406

页数：6

共 50 条

[1] Development and Validation of an HPLC-MS/MS Analytical Method for Quantitative Determination of Acetylsalicylic Acid and its Metabolite Salicylic Acid in Human Blood Plasma
K. M. Tserkovnaya
E. V. Flisyuk
V. N. Shabrov
Pharmaceutical Chemistry Journal, 2022, 55 : 1401 - 1406
[2] New HPLC-MS method for quantitative determination of apovincaminic acid in human plasma
Vlase, L
Imre, S
Leucuta, S
JOURNAL OF SEPARATION SCIENCE, 2006, 29 (03) : 385 - 389
[3] Quantitative determination of duloxetine and its metabolite in rat plasma by HPLC-MS/MS
Chae, Jung-woo
Baek, Hyun-moon
Kim, Sang Kyum
Kang, Ho-il
Kwon, Kwang-il
BIOMEDICAL CHROMATOGRAPHY, 2013, 27 (08) : 953 - 955
[4] Validation and Application of an Rapid HPLC-MS Method for the Determination of Salvianic Acid A in Human Plasma
Feng, Limin
Fu, Shujun
Du, Wuxun
Wang, Baohe
Li, Lifeng
Zhu, Mingdan
Liu, Changyu
Zhang, Jianping
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2015, 53 (05) : 771 - 777
[5] Development of an HPLC-MS/MS Method for Quantitative Determination of Rivaroxaban in Human Blood Serum
T. A. Rodina
E. S. Mel’nikov
A. A. Aksenov
S. A. Belkov
A. V. Sokolov
A. B. Prokof’ev
G. V. Ramenskaya
Pharmaceutical Chemistry Journal, 2018, 52 : 372 - 377
[6] DEVELOPMENT OF AN HPLC-MS/MS METHOD FOR QUANTITATIVE DETERMINATION OF RIVAROXABAN IN HUMAN BLOOD SERUM
Rodina, T. A.
Mel'nikov, E. S.
Aksenov, A. A.
Belkov, S. A.
Sokolov, A. V.
Prokof'ev, A. B.
Ramenskaya, G. V.
PHARMACEUTICAL CHEMISTRY JOURNAL, 2018, 52 (04) : 372 - 377
[7] Determination of glycyrrhetic acid in human plasma by HPLC-MS method and investigation of its pharmacokinetics
Zhao, W. -J.
Wang, B. -J.
Wei, C. -M.
Yuan, G. -Y.
Bu, F. -L.
Guo, R. -C.
JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS, 2008, 33 (03) : 289 - 294
[8] Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma
Liu, Gangyi
Dong, Chunxia
Shen, Weiwei
Lu, Xiaopei
Zhang, Mengqi
Gui, Yuzhou
Zhou, Qinyi
Yu, Chen
ACTA PHARMACEUTICA SINICA B, 2016, 6 (01) : 55 - 63
[9] Development and Validation of a HPLC-MS/MS Method for the Determination of Azelnidipine in Human Plasma and Application to a Bioequivalence Study
Zhang, Yunyun
Chen, Xuwang
Yuan, Guiyan
Zhang, Rui
Wang, Benjie
Guo, Ruichen
LATIN AMERICAN JOURNAL OF PHARMACY, 2016, 35 (03): : 434 - 439
[10] Development and Validation of an HPLC-MS/MS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma
He, Guodong
Mai, Liping
Wang, Xipei
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY, 2018, 2018

← 1 2 3 4 5 →