Establishment and characterization of cell lines, MeMS and MeFS, derived from spontaneous rat mesotheliomas

被引：3

作者：

Shibuya, K ^{[1
]}

Tajima, M ^{[1
]}

Saitoh, T ^{[1
]}

Shibuya, N ^{[1
]}

Kizaki, H ^{[1
]}

Yamate, J ^{[1
]}

Nunoya, T ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT VET PATHOL, SAKAI, OSAKA 593, JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1996年 / 58卷 / 07期

关键词：

cell line; mesothelioma; rat;

D O I：

10.1292/jvms.58.693

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

New cell lines, designated MeMS and MeFS, were established from the epithelial type of mesotheliomas occurring spontaneously in aged male and female Fischer 344 rats, respectively. Morphologically, MeMS and MeFS expressed the epithelial phenotype. Coexpression of keratin and vimentin in both cell lines was confirmed by the inmunocytochemistry, one-dimensional gel electrophoresis and immunoblotting for intermediate filament proteins. Hyaluronic acid synthesis of both MeMS and MeFS was demonstrated by the analysis using high-performance liquid chromatography. MeMS and MeFS seem to be useful models for studying the biological nature of rat mesotheliomas.

引用

页码：693 / 697

页数：5

共 24 条

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