DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis

被引:9
作者
Corvalan, F. H. [1 ]
Sampaio, R. N. R. [2 ]
Brustoloni, Y. M. [3 ]
Andreotti, R. [4 ]
Lima Junior, M. S. C. [5 ]
机构
[1] Univ Fed Mato Grosso do Sul, Fac Odontol, Sch Odontol, Dept Special & Community Dent, Campo Grande, MS, Brazil
[2] Univ Brasilia, Sch Med, Dept Clin Med, Dermatol Lab, Brasilia, DF, Brazil
[3] Univ Fed Mato Grosso do Sul, Dept Pediat, Sch Med, Campo Grande, MS, Brazil
[4] EMBRAPA Beef Cattle, Mol Biol Lab, Campo Grande, MS, Brazil
[5] Univ Fed Mato Grosso do Sul, Doctoral Program Infect & Parasit Dis, Campo Grande, MS, Brazil
关键词
cutaneous leishmaniasis; saliva; Leishmania; polymerase chain reaction; non-invasive diagnosis; POLYMERASE-CHAIN-REACTION; DIAGNOSIS; TRANSMISSION; PCR;
D O I
10.1590/S1678-91992011000100013
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is necessary for the diagnosis of many diseases in the future, especially infectious and parasitic ones.
引用
收藏
页码:98 / 102
页数:5
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