Arsanilic acid modified superparamagnetic iron oxide nanoparticles for Purification of alkaline phosphatase from hen's egg yolk

被引：14

作者：

Farzi-Khajeh, Hamed ^{[1
]}

Safa, Kazem D. ^{[1
]}

Dastmalchi, Siavoush ^{[2
,3
]}

机构：

[1] Univ Tabriz, Fac Chem, Organosilicon Res Lab, Tabriz, Iran

[2] Tabriz Univ Med Sci, Biotechnol Res Ctr, Tabriz, Iran

[3] Tabriz Univ Med Sci, Sch Pharm, Tabriz, Iran

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2017年 / 1061卷

关键词：

Affinity purification; Alkaline phosphatase; Arsanilic acid; Hen's egg yolk; Magnetic nanocarriers; MAGNETIC NANOPARTICLES; CARBOLIGATION REACTIVITY; COVALENT IMMOBILIZATION; BENZALDEHYDE LYASE; MICROSPHERES; CHROMATOGRAPHY; FABRICATION; EXTRACTION; SEPARATION; PROTEINS;

D O I：

10.1016/j.jchromb.2017.06.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies of magnetic carrier technology have focused on its applications in separation and purification technologies, due to easy separation of the target from the reaction medium by applying an external magnetic field. In the present study, Fe3O4 superparamagnetic nanoparticles were prepared to utilize a chemical co-precipitation method, then the surfaces of the nanoparticles were modified with arsanilic acid derivatives which were used as the specific nanocarriers for the affinity purification of alkaline phosphatase from the hen's egg yolk. The six different types of magnetic nanocarriers with varied lengths of the linkers were obtained. All samples were characterized step by step and validated using FTIR, SEM, EDX, VSM and XRD analysis methods As the results were shown, the use of inflexible tags with long linkers on the surface of the nanocarrier could lead to better results for separation of alkaline phosphatase from the hen's egg yolk with 76.2% recovery and 1361.7-fold purification. The molecular weight of the purified alkaline phosphatase was estimated to be 68 kDa by SDS-PAGE. The results of this study showed that the novel magnetic nanocarriers were capable of purifying alkaline phosphatase in a practically time and cost effective way.

引用

页码：26 / 33

页数：8

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