Long-range 1H-15N heteronuclear shift correlation at natural abundance:: a tool to study benzothiazole biodegradation by two Rhodococcus strains

被引：34
|
作者：
Besse, P
Combourieu, B
Boyse, G
Sancelme, M
De Wever, H
Delort, AM [1 ]
机构：
[1] Univ Clermont Ferrand 2, Lab Synthese & Etude Syst Interet Biol, UMR 6504 CNRS, F-63177 Clermont Ferrand, France
[2] Katholieke Univ Leuven, Lab Soil Fertil & Soil Biol, B-3001 Heverlee, Belgium
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 04期
关键词：
D O I：
10.1128/AEM.67.4.1412-1417.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of Rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by H-1 nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range H-1-N-15 heteronuclear shift correlation without any previous N-15 enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole.
引用
收藏
页码：1412 / 1417
页数：6
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