Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome

被引:3
作者
Tarszabo, Robert [1 ]
Banyai, Balint [2 ]
Ruisanchez, Eva [3 ]
Peterffy, Borbala [2 ]
Korsos-Novak, Agnes [4 ]
Lajtai, Krisztina [2 ,5 ]
Sziva, Reka Eszter [2 ,5 ]
Gerszi, Dora [2 ,5 ]
Hosszu, Adam [6 ]
Benko, Rita [2 ]
Benyo, Zoltan [3 ]
Horvath, Eszter Maria [2 ]
Masszi, Gabriella [7 ]
Varbiro, Szabolcs [5 ]
机构
[1] Markusovszky Lajos Univ Teaching Hosp, Dept Obstet & Gynecol, Markusovszky Lajos St 5, H-9700 Szombathely, Hungary
[2] Semmelweis Univ, Dept Physiol, Tuzolto St 37-47, H-1094 Budapest, Hungary
[3] Semmelweis Univ, Dept Translat Med, Tuzolto St 37-47, H-1094 Budapest, Hungary
[4] Hetenyi Geza Hosp, Dept Pathol, Toszegi St 21, H-5000 Szolnok, Hungary
[5] Semmelweis Univ, Dept Obstet & Gynecol, Ulloi St 78-A, H-1082 Budapest, Hungary
[6] Semmelweis Univ, Dept Pediat 1, H-1082 Budapest, Hungary
[7] Natl Inst Psychiat & Addict, Dept Internal Med, Lehel St 59-61, H-1135 Budapest, Hungary
关键词
polycystic ovary syndrome; vitamin D deficiency; rat model; estradiol relaxation; testosterone treatment; PHARMACOLOGICAL REACTIVITY; INSULIN-RESISTANCE; ESTROGEN-RECEPTOR; AORTIC RINGS; D DEFICIENCY; WOMEN; CYCLOOXYGENASE-2; HYPERANDROGENISM; PREVALENCE;
D O I
10.3390/ijms22179404
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined the vasoactive effect of estradiol in a rat model of early PCOS and the influence of vitamin D deficiency (VDD). We created a model of chronic hyperandrogenism and VDD in adolescent female Wistar rats (N = 46) with four experimental groups: vitamin D supplemented (T-D+), VDD (T-D-), hyperandrogenic and vitamin D supplemented (T+D+), and hyperandrogenic and VDD (T+D-). T+ groups received an 8-week-long transdermal Androgel treatment, D-animals were on vitamin D-reduced diet and D+ rats were supplemented orally with vitamin D3. Estrogen-induced vasorelaxation of thoracic aorta segments were measured with a wire myograph system with or without the inhibition of endothelial nitric oxide synthase (eNOS) or cyclooxygenase-2 (COX-2). The distribution of estrogen receptor (ER), eNOS and COX-2 in the aortic wall was assessed by immunohistochemistry. VDD aortas showed significantly lower estradiol-induced relaxation independently of androgenic status that was further decreased by COX-2 inhibition. COX-2 inhibition failed to alter vessel function in D+ rats. Inhibition of eNOS abolished the estradiol-induced relaxation in all groups. Changes in vascular function in VDD were accompanied by significantly decreased ER and eNOS staining. Short-term chronic hyperandrogenism failed to, but VDD induced vascular dysfunction, compromised estrogen-dependent vasodilatation and changes in ER and eNOS immunostaining.
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页数:12
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