A trypsin-like platelet protease propagates protease-activated receptor-1 cleavage and platelet activation

被引:28
作者
Ofosu, FA [1 ]
Freedman, J
Dewar, L
Song, YQ
Fenton, JW
机构
[1] Canadian Red Cross Soc, Blood Serv, Hamilton, ON L8N 1H8, Canada
[2] McMaster Univ, Dept Pathol, Hamilton, ON L8N 3Z5, Canada
[3] Univ Toronto, St Michaels Hosp, Toronto, ON M5B 1W8, Canada
[4] Univ Toronto, Dept Med, Toronto, ON M5B 1W8, Canada
[5] New York State Dept Hlth, Albany, NY 12201 USA
关键词
D O I
10.1042/bj3360283
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protease-activated receptor-1 (PAR-1) is a G-protein-linked receptor on platelets and perivascular cells activated by alpha-thrombin and the PAR-1-activating peptide, SFLLRN. alpha-Thrombin activates PAR-1 by cleaving it at R-41-S-42 to release the 41-residue peptide TR(1-41). Unexpectedly, platelet activation with SFLLRN was also associated with PAR-1 cleavage and the release of TR(1-41). Both PAR-1 cleavage and platelet activation resulting from SFLLRN addition to platelets were markedly inhibited by the serine protease inhibitor 4,2-(aminoethyl)benzene sulphonylfluoride . HCl (pefabloc SC) and soybean trypsin inhibitor, but not by inhibitors of calpain, cysteine proteases or metalloproteases. Thus, a trypsin-like platelet protease propagates SFLLRN-dependent PAR-1 cleavage and platelet activation.
引用
收藏
页码:283 / 285
页数:3
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