Trypanosoma evansi: Adenosine deaminase activity in the brain of infected rats

被引：15

作者：

Da Silva, Aleksandro S. ^{[1
]}

Belle, Luziane P. ^{[2
]}

Bitencourt, Paula E. R. ^{[2
]}

Garcia Perez, Herakles A. ^{[3
]}

Thome, Gustavo R. ^{[2
]}

Costa, Marcio M. ^{[4
]}

Oliveira, Camila B. ^{[1
]}

Teixeira, Marta M. G. ^{[3
]}

Moretto, Maria B. ^{[2
]}

Mazzanti, Cinthia M. ^{[4
]}

Lopes, Sonia T. A. ^{[4
]}

Monteiro, Silvia G. ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Dept Microbiol & Parasitol, BR-97105900 Santa Maria, RS, Brazil

[2] Univ Fed Santa Maria, Dept Clin Anal & Toxicol, BR-97105900 Santa Maria, RS, Brazil

[3] Univ Sao Paulo, Dept Parasitol, BR-05508 Sao Paulo, Brazil

[4] Univ Fed Santa Maria, Dept Small Anim, BR-97105900 Santa Maria, RS, Brazil

来源：

EXPERIMENTAL PARASITOLOGY | 2011年 / 127卷 / 01期

关键词：

Trypanosoma evansi; Adenosine deaminase activity; Adenosine; Brain; Rats; NERVOUS-SYSTEM; PURINE; METABOLISM; EQUIPERDUM; RESISTANCE; REGULATOR; AFFINITY; BRUCEI; HORSES; DNA;

D O I：

10.1016/j.exppara.2010.07.010

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The study was undertaken to evaluate changes in the activity of adenosine deaminase (ADA) in brains of rats infected by Trypanosoma evansi. Each rat was intraperitoneally infected with 10(6) trypomastigotes either suspended in fresh (group A; n = 13) and cryopreserved blood (group B; n = 13). Thirteen animals were used as control (group C). ADA activity was estimated in the cerebellum, cerebral cortex, striatum and hippocampus. No differences (P > 0.05) in ADA activity were observed in the cerebellum between infected and non-infected animals. Significant (P < 0.05) reductions in ADA activity occurred in cerebral cortex in acutely (day 4 post-infection; PI) and chronically (day 20 PI) infected rats. ADA activity was significantly (P < 0.05) decreased in the hippocampus in acutely infected rats, but significantly (P < 0.05) increased in the chronically infected rats. Significant (P < 0.05) reductions in ADA activity occurred in the striatum of chronically infected rats. Parasites could be found in peripheral blood and brain tissue through microscopic examination and PCR assay, respectively, in acutely and chronically infected rats. The reduction of ADA activity in the brain was associated with high levels of parasitemia and anemia in acute infections. Alterations in ADA activity of the brain in T. evansi-infected rats may have implications for pathogenesis of the disease. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：173 / 177

页数：5

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