Melatonin Alleviates Renal Injury in Mouse Model of Sepsis

被引：21

作者：

Chen, Liyang ^{[1
]}

Han, Zhijian ^{[2
]}

Shi, Zhiguang ^{[1
]}

Liu, Chao ^{[3
]}

Lu, Qiulun ^{[1
]}

机构：

[1] Nanjing Med Univ, Sch Pharm, Key Lab Cardiovasc & Cerebrovasc Med, Nanjing, Peoples R China

[2] Nanjing Med Univ, Affiliated Hosp 1, Dept Urol, Nanjing, Peoples R China

[3] Hubei Univ Sci & Technol, Hubei Key Lab Diabet & Angiopathy, Xianning, Peoples R China

来源：

FRONTIERS IN PHARMACOLOGY | 2021年 / 12卷

基金：

中国国家自然科学基金;

关键词：

melatonin; sepsis; ROS (reactive oxygen species); acute kidney injury; renal dysfunction; ACUTE KIDNEY INJURY; RATS;

D O I：

10.3389/fphar.2021.697643

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Melatonin (N-acetyl-5-methoxytryptamine; MLT) has been shown to have a renal-protective effect against kidney injury. However, the mechanisms underlying the protective role of MLT in sepsis-induced renal injury are yet to be revealed. In this study, MLT alleviated renal dysfunction with the increase of BUN (blood urea nitrogen) and SCR (serum creatinine) and reduction of fibrosis in the CLP (cecal ligation puncture) model. RNA-seq analysis showed that MLT repressed the oxidant stress in response to kidney injury. Our in vitro study showed that MLT suppresses LPS-induced accumulation of ROS (reactive oxygen species) production via SOD2 downregulation and Nox4 upregulation in HK-2 cells. Furthermore, we found that MLT alleviated the inflammatory response, with the mRNA-level reduction of Il-1 alpha, Il-1 beta, Mcp-1, and Tgf-beta 1. Taken together, in evaluating the therapeutic effect of MLT on sepsis-induced acute kidney injury, the results showed that MLT alleviated renal damage by regulating the production of ROS.

引用

页数：8

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