Bacillus camelliae sp nov., isolated from Pu'er tea

A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1(T), was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1(T) was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletonii LMG 18435(T) (98.4 %), Bacillus acidicola DSM 14745(T) (97.6 %), Bacillus paralicheniformis KACC 18426(T) (97.2 %) and Bacillus ginsengihumi KCTC 13944(T) (96.7 %). The fatty acid profile containing the major fatty acids, iso-C-15 : 0, anteiso-C-15 : 0 and anteiso-C-17 : 0 supported the allocation of strain 7578-1(T) to the genus Bacillus. The strain had a cell-wall type A1 gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-7 (95 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid. The average nucleotide identity values between strain 7578-1(T) and its most closely related species were 67.8-82.4% by OrthoANIu analysis. The DNA-DNA relatedness value between strain 7578-1(T) and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1(T) represented a novel species in the genus Bacillus. The DNA G+C content of strain 7578-1(T) was 36.0 mol%. On the basis of the presented polyphasic evidence, strain 7578-1(T) is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp. nov. The type strain is 7578-1(T) (=CGMCC 1.15374(T) =KCTC 33845(T)).