Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

被引:26
作者
Basiri, Hamideh [1 ,4 ]
Mehrizi, Ali Abouei [1 ]
Ghaee, Azadeh [1 ]
Farokhi, Mehdi [5 ]
Chekini, Mahshid [4 ]
Kumacheva, Eugenia [2 ,3 ]
机构
[1] Univ Tehran, Fac New Sci & Technol, Dept Life Sci Engn, Tehran 1439957131, Iran
[2] Univ Toronto, Dept Chem, Inst Biomat & Biomed Engn, Toronto, ON M5S 3H6, Canada
[3] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3H6, Canada
[4] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada
[5] Pasteur Inst Iran, Natl Cell Bank Iran, Tehran 1316943551, Iran
基金
加拿大自然科学与工程研究理事会;
关键词
GRAPHENE QUANTUM DOTS; FACTOR DELIVERY; SERUM-ALBUMIN; CITRIC-ACID; VEGF; DOXORUBICIN; NANODOTS; THERANOSTICS; MATRICES; RELEASE;
D O I
10.1021/acs.langmuir.9b03980
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
One of the challenges of using growth factors for tissue regeneration is to monitor their biodistributions and delivery to injured tissues for minimally invasive detection. In the present study, tracking of human vascular endothelial growth factor (VEGF) was achieved by chemically linking it to photoluminescent carbon dots (CDs). Carbon dots were synthesized by the hydrothermal method and, subsequently, conjugated with VEGF using carbodiimide coupling. ELISA and western blot analysis revealed that VEGF-conjugated CDs preserve the binding affinity of VEGF to its antibodies. We also show that VEGF-conjugated CDs maintain the functionality of VEGF for tube formation and cell migration. The VEGF-conjugated CDs were also used for in vitro imaging of human umbilical vein endothelial cells. The results of this work suggest that cell-penetrating VEGF-conjugated CDs can be used for growth factor protein tracking in therapeutic and tissue engineering applications.
引用
收藏
页码:2893 / 2900
页数:8
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