Differential expression of the Wnt/β-catenin pathway in the genital tubercle (GT) of fetal male rat following maternal exposure to di-n-butyl phthalate (DBP)

被引：24
作者：
Zhang, Li-Feng [1 ]
Qin, Chao [1 ]
Wei, Yun-Fei [2 ]
Wang, Yong [1 ]
Chang, Jun-Kai [3 ]
Mi, Yuan-Yuan [1 ]
Ma, Long [2 ]
Jiang, Jun-Tao [4 ]
Feng, Ning-Han [1 ]
Wang, Zeng-Jun [1 ]
Zhang, Wei [1 ]
机构：
[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Urol, Nanjing 210029, Peoples R China
[2] Jiangsu Prov Hosp TCM, Dept Urol, Nanjing, Peoples R China
[3] Henan Univ, Huaihe Hosp, Dept Urol, Kaifeng, Peoples R China
[4] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 1, Dept Urol, Shanghai 200030, Peoples R China
来源：
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE | 2011年 / 57卷 / 05期
基金：
中国国家自然科学基金;
关键词：
beta-catenin; di-n-butyl phthalate; genital tubercle; hypospadias; male rats; IN-UTERO EXPOSURE; FACTOR-KAPPA-B; EXTERNAL GENITALIA; BETA-CATENIN; PROSTATE-CANCER; SONIC HEDGEHOG; CELL-SURVIVAL; STEM-CELLS; HYPOSPADIAS; ANDROGEN;
D O I：
10.3109/19396368.2011.577509
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
Di-n-butyl phthalate (DBP) is one of the most abundantly produced endocrine disruptors that leaches out from polyvinyl chloride plastics and can cause hypospadias in male rats during maternal exposure. The objective of this study was to first explore the roles of Wnt/beta-catenin pathway in the fetal rat genital tubercle (GT) following in-utero exposure to DBP. Timed-pregnant rats were given DBP by gastric intubation at a dose of 750 mg/kg body weight (bw)/day from gestation day (GD) 14 to GD18 to establish a rat model of hypospadias. On GD19, genital tubercle down-regulation of beta-catenin, Phospho-GSK-3 beta, and up-regulation of GSK-3 beta (glycogen synthase kinase-3 beta), NF kappa B in fetal male rats was observed by western blot analysis. beta-catenin was located in the urethral plate epithelium (UPE). Immunochemistry showed that the relative expression of beta-catenin decreased in the DBP-treated fetal rat GT compared to the normal control. These findings, for the first time, indicate that DBP may affect the development of GT by down-regulating the Wnt/beta-catenin pathway in fetal male rats.
引用
收藏
页码：244 / 250
页数：7
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