Topical delivery of retinyl ascorbate

被引:6
作者
Abdulmajed, Kasem [1 ]
Heard, Charles M. [1 ]
机构
[1] Cardiff Univ, Welsh Sch Pharm, Cardiff CF10 3XF, Wales
关键词
retinyl ascorbate; Co-drug; retinoic acid; ascorbic acid; Franz diffusion cell; tape stripping; follicle sealing; shunt route; skin stretching;
D O I
10.1159/000111135
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
This influence of skin stretching and hair follicle sealing on the delivery of retinyl ascorbate (RA-AsA) to the epidermis was probed in vitro. Porcine ear skin was subjected to stretching by 2 and 4 mm (3.3 and 6.7%, respectively); the hair follicles of other skin sections were located and painstakingly sealed using adhesive. After mounting in Franz cells the skin was dosed with 100 mu l of 2.5 mM RA-AsA in methanol/PBS with water as receptor phase. After 24 h the diffused areas were subjected to tape stripping, and the amount of RA-AsA was determined in 5 groups of 9 strips. Statistical analysis of the resulting depth profiles showed that there was no statistical difference between unstretched skin and the skin that had the follicles sealed across the 5 depth bands. Between 0 and 2 mm stretch there were generally significant differences; between 0 and 4 mm p < 0.001 was obtained at each depth. The data from this limited exercise suggest that in native skin the follicular route does not contribute to dermal absorption, but when disturbed (as when stretched) follicular delivery can substantially increase drug delivery into the skin by up to approximately 20-40%. Skin stretching becomes difficult beyond about 7%. Copyright (C) 2007 S. Karger AG, Basel.
引用
收藏
页码:46 / 49
页数:4
相关论文
共 15 条
[1]   Topical delivery of retinyl ascorbate co-drug - 2. Comparative skin tissue and keratin binding studies [J].
Abdulmajed, K ;
Heard, CM ;
McGuigan, C ;
Pugh, WJ .
SKIN PHARMACOLOGY AND PHYSIOLOGY, 2004, 17 (06) :274-282
[2]   Topical delivery of retinyl ascorbate co-drug - 1. Synthesis, penetration into and permeation across human skin [J].
Abdulmajed, K ;
Heard, CM .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2004, 280 (1-2) :113-124
[3]   Drug delivery routes in skin: a novel approach [J].
Barry, BW .
ADVANCED DRUG DELIVERY REVIEWS, 2002, 54 :S31-S40
[4]   Lipid vesicles and other colloids as drug carriers on the skin [J].
Cevc, G .
ADVANCED DRUG DELIVERY REVIEWS, 2004, 56 (05) :675-711
[5]   Binding of primaquine to epidermal membranes and keratin [J].
Heard, CM ;
Monk, BV ;
Modley, AJ .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2003, 257 (1-2) :237-244
[6]   FOLLICLES PLAY AN IMPORTANT ROLE IN PERCUTANEOUS-ABSORPTION [J].
ILLEL, B ;
SCHAEFER, H ;
WEPIERRE, J ;
DOUCET, O .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1991, 80 (05) :424-427
[7]   Porcine ear skin:: an in vitro model for human skin [J].
Jacobi, Ute ;
Kaiser, Marco ;
Toll, Rani ;
Mangelsdorf, Susanne ;
Audring, Heike ;
Otberg, Nina ;
Sterry, Wolfram ;
Lademann, Juergen .
SKIN RESEARCH AND TECHNOLOGY, 2007, 13 (01) :19-24
[8]   Innovative liposomes as a transfollicular drug delivery system: Penetration into porcine hair follicles [J].
Jung, Sascha ;
Otberg, Nina ;
Thiede, Gisela ;
Richter, Heike ;
Sterry, Wolfram ;
Panzner, Steffen ;
Lademann, Juergen .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2006, 126 (08) :1728-1732
[9]   Transfollicular drug delivery - Is it a reality? [J].
Meidan, VM ;
Bonner, MC ;
Michniak, BB .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2005, 306 (1-2) :1-14
[10]   Investigation of differences in follicular penetration of particle- and nonparticle-containing emulsions by laser scanning microscopy [J].
Ossadnik, M. ;
Richter, H. ;
Teichmann, A. ;
Koch, S. ;
Schaefer, U. ;
Wepf, R. ;
Sterry, W. ;
Lademann, J. .
LASER PHYSICS, 2006, 16 (05) :747-750