Redox status of equine seminal plasma reflects the pattern and magnitude of DNA damage in sperm cells

被引：24

作者：

Wnuk, Maciej ^{[1
]}

Lewinska, Anna ^{[2
]}

Oklejewicz, Bernadetta ^{[1
]}

Bartosz, Grzegorz ^{[2
]}

Tischner, Marek ^{[3
]}

Bugno-Poniewierska, Monika ^{[4
]}

机构：

[1] Univ Rzeszow, Dept Genet, PL-35959 Rzeszow, Poland

[2] Univ Rzeszow, Dept Biochem & Cell Biol, PL-35959 Rzeszow, Poland

[3] Univ Agr, Dept Anim Reprod & Anat, PL-30059 Krakow, Poland

[4] Natl Res Inst Anim Prod, Dept Immuno & Cytogenet, Krakow, Poland

来源：

THERIOGENOLOGY | 2010年 / 74卷 / 09期

关键词：

Semen; Spermatozoa; Equine; Total antioxidant capacity; DNA damage; HUMAN SPERMATOZOA; OXIDATIVE STRESS; SUPEROXIDE-DISMUTASE; STALLION SPERMATOZOA; CRYOPRESERVATION; SEMEN; FRAGMENTATION; APOPTOSIS; MEN; REPRODUCTION;

D O I：

10.1016/j.theriogenology.2010.07.007

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antioxidant status of seminal plasma from 23 stallions was evaluated We found a negative correlation between total antioxidant capacity (ABTS(center dot+) decolorization assay) and thiol content of seminal plasma, and sperm DNA damage (8-oxoG immunostaining TUNEL reaction comet assay) Low seminal redox status was the strongest correlated with 8-oxoG level which may Indicate that seminal total antioxidant capacity influences mainly the formation of single strand DNA breaks in sperm cells Since inter-individual differences in seminal antioxidant status were reported we postulated that the redox status of seminal plasma may be an additional important parameter, both with sperm quantitative and morphological analysis for evaluation of equine semen quality (C) 2010 Elsevier Inc All rights reserved

引用

页码：1677 / 1684

页数：8

共 40 条

[1] The role of free radicals and antioxidants in reproduction [J].