Factors affecting measurement of channel thickness in asymmetrical flow field-flow fractionation

被引：21

作者：

Dou, Haiyang ^{[1
,2
]}

Jung, Euo Chang ^{[3
]}

Lee, Seungho ^{[2
]}

机构：

[1] Hebei Univ, Coll Basic Med Sci, Baoding 071002, Peoples R China

[2] Hannam Univ, Dept Chem, Taejon 305811, South Korea

[3] Korea Atom Energy Res Inst, Nucl Chem Res Ctr, Taejon 305353, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 2015年 / 1393卷

基金：

新加坡国家研究基金会;

关键词：

Asymmetrical flow field-flow fractionation; Channel thickness determination; Sample-membrane interaction; Steric effect; HYDRODYNAMIC RADIUS DETERMINATION; DYNAMIC LIGHT-SCATTERING; NANOPARTICLES; SYSTEM; CHROMATOGRAPHY; OPTIMIZATION; DISPERSIONS; TRANSITION; SEPARATION; RETENTION;

D O I：

10.1016/j.chroma.2015.03.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Asymmetrical flow field-flow fractionation (AF4) has been considered to be a useful tool for simultaneous separation and characterization of polydisperse macromolecules or colloidal nanoparticles. AF4 analysis requires the knowledge of the channel thickness (w), which is usually measured by injecting a standard with known diffusion coefficient (D) or hydrodynamic diameter (d(h)). An accurate w determination is a challenge due to its uncertainties arising from the membrane's compressibility, which may vary with experimental condition. In the present study, influence of factors including the size and type of the standard on the measurement of w was systematically investigated. The results revealed that steric effect and the particles membrane interaction by van der Waals or electrostatic force may result in an error in w measurement. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：115 / 121

页数：7

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