Optimization of Supercritical Fluid Consecutive Extractions of Fatty Acids and Polyphenols from Vitis Vinifera Grape Wastes

被引：37

作者：

Aizpurua-Olaizola, Oier ^{[1
]}

Ormazabal, Markel ^{[1
]}

Vallejo, Asier ^{[1
]}

Olivares, Maitane ^{[1
]}

Navarro, Patricia ^{[1
]}

Etxebarria, Nestor ^{[1
]}

Usobiaga, Aresatz ^{[1
]}

机构：

[1] Univ Basque Country UPV EHU, Dept Analyt Chem, Leioa 48940, Spain

来源：

JOURNAL OF FOOD SCIENCE | 2015年 / 80卷 / 01期

关键词：

fatty acids; optimization; polyphenols; supercritical fluid extraction; wine wastes; CHROMATOGRAPHY-MASS SPECTROMETRY; IN-VITRO; SEED OIL; LIQUID; INDUSTRY; CO2;

D O I：

10.1111/1750-3841.12715

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, supercritical fluid extraction has been successfully applied to a sequential fractionation of fatty acids and polyphenols from wine wastes (2 different vitis vinifera grapes). To this aim, in a 1st step just fatty acids were extracted and in a 2nd one the polyphenols. The variables that affected to the extraction efficiency were separately optimized in both steps following an experimental design approach. The effect of extraction temperature flow, pressure, and time were thoroughly evaluated for the extraction of fatty acids, whereas the addition of methanol was also considered in the case of the polyphenols extraction. A quantitative extraction with high efficiency was achieved at a very short time and low temperatures. Concerning quantification, fatty acids were determined by means of gas chromatography coupled to mass spectrometry after a derivatization step, whereas the polyphenols were analyzed by means of high performance liquid chromatography coupled to tandem mass spectrometry and the Folin-Ciocalteu method.

引用

页码：E101 / E107

页数：7

共 30 条

[1] Supercritical Extraction from Vinification Residues: Fatty Acids, α-Tocopherol, and Phenolic Compounds in the Oil Seeds from Different Varieties of Grape [J].