Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation

被引：17

作者：

Wu, Shao-Chun ^{[1
,2
]}

Kuo, Pao-Jen ^{[2
,3
]}

Rau, Cheng-Shyuan ^{[2
,4
]}

Huang, Lien-Hung ^{[2
,4
]}

Lin, Chia-Wei ^{[2
,3
]}

Wu, Yi-Chan ^{[2
,3
]}

Wu, Chia-Jung ^{[2
,3
]}

Tsai, Chia-Wen ^{[2
,3
]}

Hsieh, Ting-Min ^{[2
,5
]}

Liu, Hang-Tsung ^{[2
,5
]}

Huang, Chun-Ying ^{[2
,5
]}

Hsieh, Ching-Hua ^{[2
,3
,6
]}

机构：

[1] Kaohsiung Chang Gung Mem Hosp, Dept Anesthesiol, Kahosiung 83301, Taiwan

[2] Chang Gung Univ, Coll Med, Kahosiung 83301, Taiwan

[3] Kaohsiung Chang Gung Mem Hosp, Dept Plast & Reconstruct Surg, Kahosiung 83301, Taiwan

[4] Kaohsiung Chang Gung Mem Hosp, Dept Neurosurg, Kahosiung 83301, Taiwan

[5] Kaohsiung Chang Gung Mem Hosp, Dept Trauma Surg, Kahosiung 83301, Taiwan

[6] Chang Gung Mem Hosp, Ctr Vascularized Composite Allotransplantat, Taoyuan 33305, Taiwan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 16期

关键词：

angiogenesis; endothelial cell; exosome; adipose-derived stem cells; lipopolysaccharide; proteomic analysis; cAMP response element binding protein; nuclear factor-kappa B; activating protein 1; interleukin-8; NF-KAPPA-B; GROWTH-FACTOR EXPRESSION; EXTRACELLULAR VESICLES; ENDOTHELIAL-CELLS; IL-8; PRODUCTION; TUMOR-GROWTH; INTERLEUKIN-8; SURVIVAL; CREB; EIF4E;

D O I：

10.3390/ijms22168877

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing. However, in clinical settings, wounds may be infected by various bacteria or pathogens. We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs). ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 mu g/mL LPS treatment for 24 h. The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-kappa B (NF-kappa B) signaling pathways and increased the migration of and tube formation in HUVECs. RNA interference with CREB, AP-1, or NF-kappa B1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo. An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo. In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.

引用

页数：18

共 88 条

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