In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

被引：7
作者：
Zhang, Shen [1 ]
Wu, Yonggen [1 ]
Weng, Yimin [2 ]
Xu, Zhihui [1 ]
Chen, Wenmin [3 ]
Zheng, Dahan [4 ]
Lin, Wei [5 ]
Liu, Jun [6 ]
Zhou, Ying [1 ,7 ]
机构：
[1] Wenzhou Med Univ, Affiliated Hosp 1, Reprod Med Ctr, Wenzhou, Peoples R China
[2] Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed, Wenzhou, Peoples R China
[3] Wenzhou Med Univ, Affiliated Hosp 1, Dept Obstet, Wenzhou, Peoples R China
[4] Wenzhou Med Univ, Sch Lab Med & Life Sci, Wenzhou, Peoples R China
[5] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou, Peoples R China
[6] AgriBiosci Res Ctr, Stem Cells & Genet Engn Grp, Dept Econ Dev Jobs Transport & Resources, Melbourne, Vic, Australia
[7] Wenzhou Med Univ, Dept Histol & Embryol, Wenzhou, Peoples R China
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2017年 / 130期
关键词：
Developmental Biology; Issue; 130; Rotary culture system; three-dimensional culture; simulated microgravity; ovarian follicle; alginate; in vitro; ENDOTHELIAL-CELLS; CULTURE-SYSTEM; OOCYTES; LIVE; WEIGHTLESSNESS; FERTILIZATION; MATURATION; VIVO;
D O I：
10.3791/55641
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
14 day-old mouse ovarian tissue and preantral follicles isolated from same-aged mice were incubated in a simulated microgravity culture system. We quantitatively assessed follicle survival, measured follicle and oocyte diameters, and examined ultrastructure of the oocytes produced from the system. We observed decreased follicle survival, downregulation of expressions of proliferating cell nuclear antigen and growth differentiation factor 9, as indicators for the development of granulosa cells and oocytes, respectively, and oocyte ultrastructural abnormalities under the simulated microgravity condition. The simulated microgravity experimental setup needs to be optimized to provide a model for investigation of the mechanisms involved in the oocyte/follicle in vitro development.
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页数：9
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